3020664
print

  Laminin 332 and p200 Antibodies, IgG by IIF

IGGLM332B4

Ordering Recommendation

Use to assess IgG/IgG4 antibodies to laminin 332 and laminin beta4; laminin 332 is a major component of anchoring filaments in human skin and mucosa, and laminin beta4 is a structural component of cutaneous basement membrane zone and a target antigen of p200 antibodies. Testing should be correlated with concurrent indirect immunofluorescence with split skin substrate, also known as salt split skin, showing dermal or floor pattern reactivity, Basement Membrane Zone (Epithelial) Antibodies, IgG by IIF (0092056), also part of Immunobullous Disease Antibody Panel (3001409); Basement Membrane Zone Antibody Panel (3001410); Pemphigoid Antibody Panel (0092001); and Basement Membrane Zone and Cell Surface (Epithelial) Antibodies, IgG and IgA by IIF (0090299) and with Direct Immunofluorescence, Tissue Biopsy (Cutaneous, Mucosal, Epithelial) (0092572).

If other types of blistering disease are diagnostic considerations, consider ordering the serum Immunobullous Disease Antibody Panel (3001409).

New York DOH Approval Status

This test is not New York state approved. There are no New York state-approved laboratories available. Submit a Non-Permitted Laboratory Request Form (NPL) to the NYDOH prior to collection of specimen. If NPL is approved by NYDOH, and sample is received at ARUP, testing will be performed.

Specimen Required

Patient Preparation
Collect

Plain red or serum separator tube (SST).

Specimen Preparation

Transfer 2 mL serum to an ARUP standard transport tube. (Min: 0.5 mL)

Storage/Transport Temperature

Refrigerated/ambient

Unacceptable Conditions

Hemolyzed or lipemic specimens. Plasma.

Remarks

As a general rule, serum specimens should be shipped to the laboratory as soon as possible.

Store refrigerated unless shipping promptly (within 2 hours). Transport at ambient temperature to arrive within 7 days. If 7-14 days until received in laboratory, store and ship refrigerated. If greater than 14 days, serum specimens must be stored and shipped frozen.

Stability

Ambient: 1 week; Refrigerated: 2 weeks; Frozen: Indefinitely

Methodology

Qualitative Indirect Immunofluorescence (IIF)

Performed

Varies

Reported

3-14 days

Reference Interval

Interpretive Data

Refer to report.

Compliance Category

Performed by non-ARUP Laboratory

Note

The methodology is indirect immunofluorescence (IIF) of serum with substrates consisting of transfected cells expressing whole-length recombinant three-chain laminin 332 and transfected cells expressing the laminin beta4 chain that are grown and fixed as individual substrates on cover glass called “biochips”.
Of important note, patients with anti-laminin 332 pemphigoid who develop antibodies to any of the three laminin 332 subunit chains are at risk for having an associated malignancy or developing one.
Laminin 332 previously was known as laminin 5, epiligrin, nicein, ladsin, and kalinin.
Patients with anti-p200 pemphigoid and antibodies to laminin beta4 also commonly have antibodies to laminin gamma1; ant-p200 pemphigoid was referred to previously as anti-laminin gamma1 pemphigoid.
Reports are qualitative, positive or negative (no antibody titers).
For specimens less than 0.5 mL, call the Immunodermatology Laboratory at 801-581-7139.

Hotline History

View Hotline History

 

Hotline History

Date of Change
Test Name Change
Methodology
Performance/Reported Schedule
Specimen Requirements
Reference Interval
Interpretive Data
Note
CPT Code
Component Change
Other Interface Change
New Test
Inactive
04/20/2026
X
N/A

CPT Codes

88346; 88350

Are you an ARUP Client? Click here for your pricing.

Components

Component Test Code* Component Chart Name LOINC
3020665 Laminin 332 and p200 Ab, IgG/IgG4 by IIF
3020680 EER Laminin 332/p200 Ab, IgG/IgG4 by IIF 11502-2
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • anti-epiligrin pemphigoid
  • anti-ladsin pemphigoid
  • anti-laminin 5 pemphigoid
  • anti-laminin beta4 pemphigoid
  • anti-laminin gamma1 pemphigoid
  • anti-nicein pemphigoid
  • anti-p200 pemphigoid
  • autoimmune bullous disorder
  • dermal basement membrane zone antibodies
  • epiligrin antibody pemphigoid
  • epithelial antibody-associated disease
  • floor antibodies salt split skin
  • floor basement membrane zone antibodies split skin substrate
  • indirect fluorescence assay
  • indirect fluorescent antibody (IFA)
  • indirect immunofluorescent assay
  • indirect immunofluorescence test (IIFT)
  • kalinin antibody pemphigoid
  • ladsin antibody pemphigoid
  • laminin 5 antibody pemphigoid
  • laminin 5 pemphigoid
  • laminin beta4 antibody pemphigoid
  • laminin beta4 pemphigoid
  • laminin gamma1 antibody pemphigoid
  • laminin gamma1 pemphigoid
  • laminin gamma1/beta4 antibody pemphigoid
  • laminin gamma1/beta4 pemphigoid
  • nicein antibody pemphigoid
  • p200 antibody pemphigoid
  • p200 pemphigoid
  • pemphigoid variant
  • pemphigoid variant disease
  • salt split skin floor antibodies
  • split skin floor basement membrane zone antibodies
Laminin 332 and p200 Antibodies, IgG by IIF