0092572
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Direct Immunofluorescence, Tissue Biopsy (Cutaneous, Mucosal, Epithelial)

CUTDIF
Example Reports
Enhanced Report
Negative
Positive

Ordering Recommendation

Use with serum immunobullous disease/epithelial antibody testing and separate formalin-fixed tissue histopathology for assessment of pruritic, urticarial, blistering, and/or erosive disorders. Use with separate formalin-fixed tissue histopathology for assessment of inflammatory, immune-mediated cutaneous disease.

Optimal specimen location and complementary serum testing and/or histopathology examination vary according to disease type. Note that specimen location and transport medium/fixative are different for direct immunofluorescence testing and fixed-tissue histopathology.

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation
Collect

Tissue: skin, mucosa (oral, conjunctival, genital, esophageal), other epithelium (gastrointestinal, respiratory, urinary).

Specimen Preparation

Transport tissue (optimal 4-6 mm) in Michel's medium (ARUP supply #45462) available online through eSupply using ARUP ConnectTM or call ARUP Client Services at 800-522-2787. Also acceptable: Zeus tissue fixative. Label container with transport medium type, if not an ARUP-supplied vial.

Storage/Transport Temperature

Room temperature. Also acceptable: Refrigerated.

Unacceptable Conditions

Formalin-fixed tissue. Frozen in Michels medium. Solid organs or solid organ tissue. Tissue in container of unknown or unacceptable transport medium. Tissue sections on slides, prestained or unstained.

Remarks
Stability

Ambient: 10 days; Refrigerated: 10 days; Frozen: Unacceptable

Methodology

Direct Immunofluorescence

Performed

Varies

Reported

3-9 days

Reference Interval

By report

Interpretive Data

Refer to report

Compliance Category

Performed by non-ARUP Laboratory

Note

Hotline History

View Hotline History

 

Hotline History

Date of Change
Test Name Change
Methodology
Performance/Reported Schedule
Specimen Requirements
Reference Interval
Interpretive Data
Note
CPT Code
Component Change
Other Interface Change
New Test
Inactive
01/21/2025
X
N/A

CPT Codes

88346; 88350 x5

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Components

Component Test Code* Component Chart Name LOINC
0092571 Cutaneous Direct IF, Biopsy 6471-7
2010916 EER Cutaneous Direct IF, Biopsy
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • atypical epithelial antibody-associated disease
    basement membrane zone antibody-associated disease
    Brunsting-Perry cicatricial pemphigoid
    Brunsting-Perry pemphigoid
    bullous disease
    bullous disease of childhood
    bullous lupus erythematosus
    bullous pemphigoid
    bullous systemic lupus erythematosus
    celiac rash
    cell surface antibody-associated disease
    childhood immunobullous disease
    chronic bullous disease
    chronic bullous disease of childhood
    cicatricial pemphigoid
    Cutaneous Direct Immunofluorescence, Biopsy
    cutaneous herpetiformis
    cutaneous lupus
    dermal basement membrane zone (floor) antibodies with split skin substrate
    dermatitis herpetiformis
    DIF
    drug hypersensitivity
    drug reaction
    endemic pemphigus
    epidermolysis bullosa acquisita
    epithelial antibody-associated disease
    epithelial basement membrane zone antibody-associated disease
    epithelial cell surface antibody associated disease
    fogo selvagem
    gluten rash
    Henoch-Schönlein purpura
    herpetiformis
    hypocomplementemic urticarial vasculitis
    ICS antibodies
    IgA mucous membrane pemphigoid
    IgA pemphigus
    IgA variant epidermolysis bullosa acquisita (EBA)
    IgA vasculitis
    IgG/IgA basement membrane zone antibodies
    IgG pemphigus variant
    IgG variant pemphigus
    immune-mediated vasculitis
    immunobullous disease of childhood
    intercellular IgG/IgA dermatosis
    intercellular substance antibodies
    intraepidermal neutrophilic IgA dermatosis
    leukocytoclastic vasculitis
    lichen planus
    lichenoid reaction
    linear IgA bullous dermatosis
    Linear IgA Bullous, Tissue
    linear IgA disease
    linear IgG/IgA bullous dermatosis
    lupus
    lupus erythematosus
    Lupus Erythematous, Tissue
    mixed epithelial antibody-associated disease
    mixed immunobullous disease of childhood
    mucous membrane pemphigoid
    ocular cicatricial pemphigoid
    pemphigoid gestationis
    pemphigoid, tissue
    pemphigoid variants
    Pemphigus
    pemphigus erythematosus
    pemphigus foliaceus
    pemphigus variant
    pemphigus vegetans
    pemphigus vulgaris
    pemphigus, tissue
    porphyria and pseudoporphyria, tissue
    porphyria, pseudoporphyria tissue
    skin immunofluorescence
    subacute cutaneous lupus erythematosus
    subcorneal pustular dermatosis
    subepithelial immunobullous disease
    urticaria
    urticarial pemphigoid
    urticarial vasculitis
    vasculitis
    vasculopathy
Direct Immunofluorescence, Tissue Biopsy (Cutaneous, Mucosal, Epithelial)