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Direct Immunofluorescence, Tissue Biopsy (Cutaneous, Mucosal, Epithelial)
Ordering Recommendation
Use with serum immunobullous disease/epithelial antibody testing and separate formalin-fixed tissue histopathology for assessment of pruritic, urticarial, blistering, and/or erosive disorders. Use with separate formalin-fixed tissue histopathology for assessment of inflammatory, immune-mediated cutaneous disease.
Optimal specimen location and complementary serum testing and/or histopathology examination vary according to disease type. Note that specimen location and transport medium/fixative are different for direct immunofluorescence testing and fixed-tissue histopathology.
New York DOH Approval Status
Specimen Required
Tissue: skin, mucosa (oral, conjunctival, genital, esophageal), other epithelium (gastrointestinal, respiratory, urinary).
Transport tissue (optimal 4-6 mm) in Michel's medium (ARUP supply #45462) available online through eSupply using ARUP Connect (TM) or call ARUP Client Services at 800-522-2787. Also acceptable: Zeus tissue fixative. Label container with transport medium type, if not an ARUP-supplied vial.
Room temperature. Also acceptable: Refrigerated.
Formalin-fixed tissue. Frozen in Michel's medium. Solid organs or solid organ tissue. Tissue in container of unknown or unacceptable transport medium. Tissue sections on slides, prestained or unstained.
Ambient: 10 days; Refrigerated: 10 days; Frozen: Unacceptable
Methodology
Direct Immunofluorescence
Performed
Varies
Reported
3-9 days
Reference Interval
By report
Interpretive Data
Refer to report
Performed by non-ARUP Laboratory
Note
Hotline History
Hotline History
CPT Codes
88346; 88350 x5
Components
| Component Test Code* | Component Chart Name | LOINC |
|---|---|---|
| 0092571 | Cutaneous Direct IF, Biopsy | 6471-7 |
| 2010916 | EER Cutaneous Direct IF, Biopsy |
Aliases
- atypical epithelial antibody-associated disease,
- basement membrane zone antibody-associated disease
