Ordering Recommendation

• Order concurrently with serum antibody testing and formalin-fixed tissue histopathology for assessment of patient with pruritic, urticarial, blistering, crusted, and/or erosive disorders, including possible pemphigoid and pemphigoid variants, pemphigus and pemphigus subtypes, dermatitis herpetiformis, epidermolysis bullous acquisita, porphyria, and pseudoporphyria.
• Order concurrently with formalin-fixed tissue histopathology for assessment of patient with inflammatory, immune-mediated cutaneous disease, including possible lupus and lupus variants, vasculitis, drug reactions, lichen planus and lichenoid reactions.
• Optimal specimen location varies according to disease type, blistering or nonblistering.​


Direct Immunofluorescence




3-7 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation

Tissue: skin, mucosa (oral, conjunctival, genital, esophageal), other epithelium (gastrointestinal, respiratory, urinary).

Specimen Preparation

Transport tissue (optimal 4-6 mm) in Michel's medium (ARUP supply #45462) available online through eSupply using ARUP Connect or call ARUP Client Services at (800) 522-2787. Also acceptable: Zeus tissue fixative.

Storage/Transport Temperature

Room temperature. Also acceptable: Refrigerated.

Unacceptable Conditions

Formalin-fixed tissue. Solid organs or solid organ tissue.


Ambient: 10 days; Refrigerated: 10 days; Frozen: Unacceptable

Reference Interval

By report

Interpretive Data

No compliance statements are in use for this test.

Hotline History
CPT Codes

88346; 88350 x5

Component Test Code* Component Chart Name LOINC
0092571 Cutaneous Direct IF, Biopsy 6471-7
2010916 EER Cutaneous Direct IF, Biopsy
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • Bullous Disease
  • Chronic Bullous Disease
  • Cutaneous Herpetiformis
  • Dermatitis Herpetiformis
  • Lichen Planus and Lichenoid
  • Linear IgA Bullous, Tissue
  • Lupus Erythematous, Tissue
  • Pemphigoid, Tissue
  • Pemphigus
  • Porphyria and Pseudoporphyria, Tissue
  • Skin Immunofluorescence
  • Urticaria
  • Urticarial Pemphigoid
  • Urticarial Vasculitis
  • Vasculitis
Cutaneous Direct Immunofluorescence, Biopsy