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Direct Immunofluorescence, Tissue Biopsy (Cutaneous, Mucosal, Epithelial)
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Ordering Recommendation
Optimal specimen location and complementary serum testing and/or histopathology examination vary according to disease type. Note that specimen location and transport medium/fixative are different for direct immunofluorescence testing and fixed-tissue histopathology.
Use with serum immunobullous disease/epithelial antibody testing and formalin-fixed tissue histopathology for assessment of pruritic, urticarial, blistering, and/or erosive disorders. Use with formalin-fixed tissue histopathology for assessment of inflammatory, immune-mediated cutaneous disease.
For more information on specimen collection requirements and diseases assessed, see the ARUP Immunobullous Disease Testing Comparison tool.
Mnemonic
Methodology
Direct Immunofluorescence
Performed
Varies
Reported
3-7 days
New York DOH Approval Status
Specimen Required
Tissue: skin, mucosa (oral, conjunctival, genital, esophageal), other epithelium (gastrointestinal, respiratory, urinary).
Transport tissue (optimal 4-6 mm) in Michel's medium (ARUP supply #45462) available online through eSupply using ARUP Connector call ARUP Client Services at (800) 522-2787. Also acceptable: Zeus tissue fixative. Label container with transport medium type, if not an ARUP-supplied vial.
Room temperature. Also acceptable: Refrigerated.
Formalin-fixed tissue. Frozen in Michels medium. Solid organs or solid organ tissue. Tissue in container of unknown or unacceptable transport medium. Tissue sections on slides, prestained or unstained.
Ambient: 10 days; Refrigerated: 10 days; Frozen: Unacceptable
Reference Interval
By report
Interpretive Data
Refer to report
Performed by non-ARUP Laboratory
Note
Hotline History
Hotline History
CPT Codes
88346; 88350 x5
Components
| Component Test Code* | Component Chart Name | LOINC |
|---|---|---|
| 0092571 | Cutaneous Direct IF, Biopsy | 6471-7 |
| 2010916 | EER Cutaneous Direct IF, Biopsy |
Aliases
- atypical epithelial antibody-associated disease
- basement membrane zone antibody-associated disease
- Bullous Disease
- bullous disease of childhood
- bullous lupus erythematosus
- bullous pemphigoid
- childhood immunobullous disease
- Chronic Bullous Disease
- chronic bullous disease of childhood
- cicatricial pemphigoid
- Cutaneous Direct Immunofluorescence, Biopsy
- Cutaneous Herpetiformis
- cutaneous lupus
- Dermatitis Herpetiformis
- drug hypersensitivity
- drug reaction
- endemic pemphigus
- epidermolysis bullosa acquisita
- epithelial antibody-associated disease
- fogo selvagem, IgA pemphigus
- Henoch-Schönlein purpura
- herpetiformis
- hypocomplementemic urticarial vasculitis
- IgA vasculitis
- immune-mediated vasculitis
- intercellular IgG/IgA dermatosis
- intercellular substance antibodies
- intraepidermal neutrophilic IgA dermatosis
- leukocytoclastic vasculitis
- lichen planus
- Lichen Planus and Lichenoid
- lichenoid
- linear IgA bullous dermatosis
- Linear IgA Bullous, Tissue
- linear IgA disease
- linear IgG/IgA bullous dermatosis
- lupus
- lupus erythematosus
- Lupus Erythematous, Tissue
- mixed epithelial antibody-associated disease
- mixed immunobullous disease of childhood
- mucous membrane pemphigoid
- ocular cicatricial pemphigoid
- pemphigoid gestationis
- pemphigoid variants
- Pemphigoid, Tissue
- Pemphigus
- pemphigus erythematosus
- pemphigus foliaceus
- pemphigus vegetans
- pemphigus vulgaris
- pemphigus, tissue
- Porphyria and Pseudoporphyria, Tissue
- porphyria, pseudoporphyria, tissue
- Skin Immunofluorescence
- subacute cutaneous lupus erythematosus
- subcorneal pustular dermatosis
- subepithelial immunobullous disease
- Urticaria
- Urticarial Pemphigoid
- Urticarial Vasculitis
- Vasculitis
- vasculopathy
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