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Direct Immunofluorescence, Tissue Biopsy (Cutaneous, Mucosal, Epithelial)
Ordering Recommendation
Optimal specimen location and complementary serum testing and/or histopathology examination vary according to disease type. Note that specimen location and transport medium/fixative are different for direct immunofluorescence testing and fixed-tissue histopathology.
Use with serum immunobullous disease/epithelial antibody testing and formalin-fixed tissue histopathology for assessment of pruritic, urticarial, blistering, and/or erosive disorders. Use with formalin-fixed tissue histopathology for assessment of inflammatory, immune-mediated cutaneous disease.
For more information on specimen collection requirements and diseases assessed, see the ARUP Immunobullous Disease Testing Comparison tool.
New York DOH Approval Status
Specimen Required
Tissue: skin, mucosa (oral, conjunctival, genital, esophageal), other epithelium (gastrointestinal, respiratory, urinary).
Transport tissue (optimal 4-6 mm) in Michel's medium (ARUP supply #45462) available online through eSupply using ARUP Connect™ or call ARUP Client Services at 800-522-2787. Also acceptable: Zeus tissue fixative. Label container with transport medium type, if not an ARUP-supplied vial.
Room temperature. Also acceptable: Refrigerated.
Formalin-fixed tissue. Frozen in Michel's medium. Solid organs or solid organ tissue. Tissue in container of unknown or unacceptable transport medium. Tissue sections on slides, prestained or unstained.
Ambient: 10 days; Refrigerated: 10 days; Frozen: Unacceptable
Methodology
Direct Immunofluorescence
Performed
Varies
Reported
3-9 days
Reference Interval
By report
Interpretive Data
Refer to report
Performed by non-ARUP Laboratory
Note
Hotline History
CPT Codes
88346; 88350 x5
Components
| Component Test Code* | Component Chart Name | LOINC |
|---|---|---|
| 0092571 | Cutaneous Direct IF, Biopsy | 6471-7 |
| 2010916 | EER Cutaneous Direct IF, Biopsy |
Aliases
- atypical epithelial antibody-associated disease
- basement membrane zone antibody-associated disease
- Brunsting-Perry cicatricial pemphigoid
- Brunsting-Perry pemphigoid
- bullous disease
- bullous disease of childhood
- bullous lupus erythematosus
- bullous pemphigoid
- bullous systemic lupus erythematosus
- celiac rash
- cell surface antibody-associated disease
- childhood immunobullous disease
- chronic bullous disease
- chronic bullous disease of childhood
- cicatricial pemphigoid
- Cutaneous Direct Immunofluorescence, Biopsy
- cutaneous herpetiformis
- cutaneous lupus
- dermal basement membrane zone (floor) antibodies with split skin substrate
- dermatitis herpetiformis
- DIF
- drug hypersensitivity
- drug reaction
- endemic pemphigus
- epidermolysis bullosa acquisita
- epithelial antibody-associated disease
- epithelial basement membrane zone antibody-associated disease
- epithelial cell surface antibody associated disease
- fogo selvagem
- gluten rash
- Henoch-Schönlein purpura
- herpetiformis
- hypocomplementemic urticarial vasculitis
- ICS antibodies
- IgA mucous membrane pemphigoid
- IgA pemphigus
- IgA variant epidermolysis bullosa acquisita (EBA)
- IgA vasculitis
- IgG pemphigus variant
- IgG variant pemphigus
- IgG/IgA basement membrane zone antibodies
- immune-mediated vasculitis
- immunobullous disease of childhood
- intercellular IgG/IgA dermatosis
- intercellular substance antibodies
- intraepidermal neutrophilic IgA dermatosis
- leukocytoclastic vasculitis
- lichen planus
- lichenoid reaction
- linear IgA bullous dermatosis
- Linear IgA Bullous, Tissue
- linear IgA disease
- linear IgG/IgA bullous dermatosis
- lupus
- lupus erythematosus
- Lupus Erythematous, Tissue
- mixed epithelial antibody-associated disease
- mixed immunobullous disease of childhood
- mucous membrane pemphigoid
- ocular cicatricial pemphigoid
- pemphigoid gestationis
- pemphigoid variants
- pemphigoid, tissue
- Pemphigus
- pemphigus erythematosus
- pemphigus foliaceus
- pemphigus variant
- pemphigus vegetans
- pemphigus vulgaris
- pemphigus, tissue
- porphyria and pseudoporphyria, tissue
- porphyria, pseudoporphyria tissue
- skin immunofluorescence
- subacute cutaneous lupus erythematosus
- subcorneal pustular dermatosis
- subepithelial immunobullous disease
- urticaria
- urticarial pemphigoid
- urticarial vasculitis
- vasculitis
- vasculopathy
