Ordering Recommendation

• Assess and monitor disease in patient with pemphigoid or epidermolysis bullosa acquisita who has positive IgG basement membrane zone (BMZ) antibodies by indirect immunofluorescence, either epidermal (roof) pattern or dermal (floor) pattern, on split skin substrate. 
• Consider ordering concurrently with Epithelial Basement Membrane Zone Antibody IgA (0092057), Bullous Pemphigoid Antigens (180 kDa and 230 kDa), IgG (0092566), and/or Collagen Type VII Antibody IgG by ELISA (2010905); alternatively order Basement Membrane Zone Antibody Panel (3001410) or Immunobullous Disease Panel, Epithelial (3001409).
• May use to screen for pemphigoid and other immunobullous diseases; however, this test will not detect IgA BMZ antibodies and has decreased sensitivity and specificity when compared with Pemphigoid Antibody Panel - Epithelial Basement Membrane Zone Antibodies, IgG and IgA, BP180 and BP230 Antibodies, IgG (0092001) and Basement Membrane Zone Antibody Panel (3001410), which includes epithelial basement membrane zone antibodies, IgG and IgA; BP180 and BP230 antibodies, IgG; and collagen type VII antibody, IgG.


Indirect Immunofluorescence
(Indirect Fluorescent Antibody)




4-9 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation

Plain red or serum separator tube (SST).

Specimen Preparation

Transfer 2 mL serum to an ARUP Standard Transport Tube. (Min: 0.5 mL)

Storage/Transport Temperature


Unacceptable Conditions

Hemolyzed or lipemic specimens.


Ambient: 1 week; Refrigerated: 2 weeks; Frozen: Indefinitely

Reference Interval

By report

Interpretive Data

No compliance statements are in use for this test.


For specimens less than 0.5 mL, call the Immunodermatology Laboratory at (866) 266-5699.

Hotline History
CPT Codes

88346; 88350

Component Test Code* Component Chart Name LOINC
0092056 Epithelial BMZ Ab, IgG
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Epithelial Basement Membrane Zone Antibody IgG