Ordering Recommendation

If pursuing antibody testing to determine autoimmune diabetes mellitus (DM), perform at least two antibody tests. In most cases, use glutamic acid decarboxylase antibody in combination with another antibody test. Other antibody tests include Glutamic Acid Decarboxylase Antibody (2001771), Insulin Antibody (0099228), and Zinc Transporter 8 Antibody (2006196). Most useful to establish autoimmune etiology in previously diagnosed type 1 DM. Do not use to differentiate type 1 DM from type 2 DM, for most cases.

 

Mnemonic
ANTI-ISLET
Methodology

Semi-Quantitative Indirect Fluorescent Antibody

Performed

Sun-Sat

Reported

1-3 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer1 mL serum to an ARUP Standard Transport Tube. (Min: 0.15 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Plasma. Contaminated, hemolyzed, or severely lipemic specimens.

Remarks
Stability

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Reference Interval

< 1:4 No antibody detected.

Interpretive Data

Islet cell antibodies (ICAs) are associated with type 1 diabetes (T1D), an autoimmune endocrine disorder. ICAs may be present years before the onset of clinical symptoms. To calculate Juvenile Diabetes Foundation (JDF) units: multiply the titer x 5 (1:8  8 x 5 = 40 JDF Units).

Compliance Category

Analyte Specific Reagent (ASR)

Note
Hotline History
N/A
CPT Codes

86341

Components
Component Test Code* Component Chart Name LOINC
0050138 Islet Cell Cytoplasmic Antibody, IgG 13927-9
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Anti-Islet Cell Antibody
  • beta cell
  • Cytoplasmic Islet Cell Ab
  • ICA
  • Islet Cell Ab IgG
Islet Cell Cytoplasmic Antibody, IgG