Red Blood Cell Antigen Genotyping, Fetal
Ordering Recommendation
Fetal genotyping for 35 red blood cell antigens and phenotypic variants to assess risk for alloimmune hemolytic disease. This test does not assess for RhD; to test for RhD, refer to RhD Gene (RHD) Copy Number, Fetal (3016640).
Methodology
Polymerase Chain Reaction (PCR)/Fluorescence Monitoring/Fragment Analysis
Performed
Varies
Reported
3-10 days
New York DOH Approval Status
Specimen Required
Fetal genotyping: Amniotic fluid
OR Cultured amniocytes: Two T-25 flasks at 80 percent confluency.
If the client is unable to culture, order test Cytogenetics Grow and Send (ARUP test code 0040182) in addition to this test and ARUP will culture upon receipt (culturing fees will apply). If you have any questions, contact ARUP's Genetics Processing at 800-522-2787 ext. 3301.
AND Maternal cell contamination specimen: Lavender (K2EDTA), pink (K2EDTA), or yellow (ACD solution A or B).
Genotyping: Transport 3 mL whole blood. (Min: 1 mL)
Amniotic fluid: Transport 10 mL amniotic fluid in a sterile container. (Min: 5 mL)
Cultured amniocytes: Transport two T-25 flasks at 80 percent confluency filled with culture media. Backup cultures must be retained at the client's institution until testing is complete.
Maternal cell contamination specimen: Transport 3 mL whole blood (Min: 1 mL)
Cultured amniocytes: CRITICAL ROOM TEMPERATURE. Must be received within 48 hours of shipment due to lability of cells.
Whole blood or maternal cell contamination specimen: Refrigerated.
Plasma or serum; collection of specimens in sodium heparin tubes. Frozen specimens in glass collection tubes.
Maternal specimen is recommended for proper test interpretation if contamination of the fetal specimen from the mother is suspected. Order Maternal Cell Contamination.
Whole blood or maternal cell contamination specimen: Ambient: 72 hours; Refrigerated: 1 week; Frozen: 1 month.
Fetal specimens: Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
Reference Interval
Interpretive Data
Refer to report
Counseling and informed consent are recommended for genetic testing. Consent forms are available online.
Laboratory Developed Test (LDT)
Note
Maternal specimen is recommended for proper test interpretation; order Maternal Cell Contamination, Maternal Specimen.
Hotline History
Hotline History
CPT Codes
0001U; 81265 Fetal Cell Contamination (FCC)
Components
Component Test Code* | Component Chart Name | LOINC |
---|---|---|
0050548 | Maternal Contamination Study Fetal Spec | 59266-7 |
0050612 | Maternal Contam Study, Maternal Spec | 66746-9 |
3001055 | Rh Antigen C/c | |
3001056 | Rh Antigen E/e | |
3001057 | Rh Antigen V/VS | |
3001058 | Kell Antigen K/k | |
3001059 | Kell Antigen Kpa/Kpb | |
3001060 | Kell Antigen Jsa/Jsb | |
3001061 | Duffy Antigen Fya/Fyb | |
3001062 | Kidd Antigen Jka/Jkb | |
3001063 | MNS Antigen MN | |
3001064 | MNS Antigen S/s/U | |
3001065 | Lutheran Antigen Lua/Lub | |
3001066 | Dombrock Antigen Doa/Dob | |
3001067 | Dombrock Antigen Hy | |
3001068 | Dombrock Antigen Joa | |
3001069 | Landsteiner-Wiener Antigen LWa/LWb | |
3001070 | Diego Antigen Dia/Dib | |
3001071 | Colton Antigen Coa/Cob | |
3001072 | Scianna Antigen Sc1/Sc2 | |
3001073 | Hemoglobin S Antigen | |
3016643 | RBC Antigen Genotyping, Fetal Specimen | 31208-2 |
3016644 | RBC Antigen Genotyping Fetal, Interp | 50398-7 |
Aliases
- Colton genotyping (Coa, Cob)
- Diego genotyping (Dia, Dib)
- Dombrock genotyping (Doa, Dob, Hy, Joa)
- Duffy genotyping (Fya, Fyb, Fyb-67C)
- Kell genotyping (K, k, Kpa, Kpb, Jsa, Jsb)
- Kidd genotyping (Jka, Jkb)
- Landsteiner-Wiener genotyping (LWa, LWb)
- Lutheran genotyping (Lua, Lub)
- MNS genotyping (M, N, S, s, U, Uvar, Uneg)
- Rh genotyping (C, c, E, e, V, VS)
- Scianna genotyping (Sc1, Sc2)