Ordering Recommendation

Use to predict the expression of red blood cell (RBC) antigen specificities, aid in the selection of antigen-negative RBCs for transfusion, assess risk for hemolytic disease of the fetus and newborn, or resolve conflicting serologic antibody results. May be used for genotyping on whole blood specimens or for fetal genotyping. This test does not assess for RhD; to test for RhD, refer to RhD Gene (RHD) Copy Number (0051368).

Mnemonic

RBC GENO

Methodology

Polymerase Chain Reaction/Fluorescence Monitoring

Performed

Varies

Reported

3-10 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation
Collect

Fetal genotyping: Amniotic fluid
OR Cultured amniocytes: Two T-25 flasks at 80 percent confluency.
If the client is unable to culture, order test Cytogenetics Grow and Send (ARUP test code 0040182) in addition to this test and ARUP will culture upon receipt (culturing fees will apply). If you have any questions, contact ARUP's Genetics Processing at 800-522-2787 ext. 3301.
WITH maternal cell contamination specimen
: Lavender (K2EDTA), Pink (K2EDTA), or Yellow (ACD Solution A or B).
OR Genotyping:
Lavender (K2EDTA), Pink (K2EDTA)

Specimen Preparation

Genotyping: Transport 3 mL whole blood. (Min: 1 mL)
Amniotic fluid:
Transport 10 mL amniotic fluid in a sterile container. (Min: 5 mL)
Cultured amniocytes: Transport two T-25 flasks at 80 percent confluency filled with culture media. Backup cultures must be retained at the client's institution until testing is complete.
Maternal cell contamination specimen:
Transport 3 mL whole blood (Min: 1 mL)

Storage/Transport Temperature

Cultured amniocytes: CRITICAL ROOM TEMPERATURE. Must be received within 48 hours of shipment due to lability of cells.
Whole blood or maternal cell contamination specimen:
Refrigerated.

Unacceptable Conditions

Plasma or serum; collection of specimens in sodium heparin tubes. Frozen specimens in glass collection tubes.

Remarks

Maternal specimen is recommended for proper test interpretation if contamination of the fetal specimen from the mother is suspected. Order Maternal Cell Contamination.

Stability

Whole blood or maternal cell contamination specimen: Ambient: 72 hours; Refrigerated: 1 week; Frozen: 1 month.
Fetal specimens:
Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable

Reference Interval

Interpretive Data

Refer to report

Counseling and informed consent are recommended for genetic testing. Consent forms are available online.

Compliance Category

FDA

Note

Hotline History

N/A

CPT Codes

0001U

Components

Component Test Code* Component Chart Name LOINC
3001054 RBC Antigen Genotyping Specimen 31208-2
3001055 Rh Antigen C/c
3001056 Rh Antigen E/e
3001057 Rh Antigen V/VS
3001058 Kell Antigen K/k
3001059 Kell Antigen Kpa/Kpb
3001060 Kell Antigen Jsa/Jsb
3001061 Duffy Antigen Fya/Fyb
3001062 Kidd Antigen Jka/Jkb
3001063 MNS Antigen MN
3001064 MNS Antigen S/s/U
3001065 Lutheran Antigen Lua/Lub
3001066 Dombrock Antigen Doa/Dob
3001067 Dombrock Antigen Hy
3001068 Dombrock Antigen Joa
3001069 Landsteiner-Wiener Antigen LWa/LWb
3001070 Diego Antigen Dia/Dib
3001071 Colton Antigen Coa/Cob
3001072 Scianna Antigen Sc1/Sc2
3001073 Hemoglobin S Antigen
3001074 RBC Antigen Genotyping Interpretation 50398-7
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • Colton genotyping (Coa, Cob)
  • Diego genotyping (Dia, Dib)
  • Dombrock genotyping (Doa, Dob, Hy, Joa)
  • Duffy genotyping (Fya, Fyb, Fyb-67C)
  • Kell genotyping (K, k, Kpa, Kpb, Jsa, Jsb)
  • Kidd genotyping (Jka, Jkb)
  • Landsteiner-Wiener genotyping (LWa, LWb)
  • Lutheran genotyping (Lua, Lub)
  • MNS genotyping (M, N, S, s, U, Uvar, Uneg)
  • Rh genotyping (C, c, E, e, V, VS)
  • Scianna genotyping (Sc1, Sc2)
Red Blood Cell Antigen Genotyping