Ordering Recommendation

Use to assess donor genotype and compare to recipient genotype prior to allogeneic hematopoietic stem cell transplant. For additional donor(s) testing, refer to Chimerism, Additional Donor (3005468). Pretransplant genotyping is required to enable posttransplant chimerism and engraftment monitoring.

Mnemonic

STR_DONOR

Methodology

Polymerase Chain Reaction/Fragment Analysis

Performed

Sun-Sat

Reported

5-9 days after receipt of corresponding Chimerism, Recipient, Pretransplant (3005449) specimen

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation
Collect

Lavender (EDTA), pink (K2EDTA), or yellow (ACD solution A or B) OR bone marrow in lavender (EDTA) OR buccal brushes from donor.

Specimen Preparation

Transport 2 mL whole blood (Min: 1 mL) OR 1 mL bone marrow (Min: 1 mL) OR 2 buccal brushes in a sterile, dry tube. (Min: 2 brushes)

Storage/Transport Temperature

Refrigerated. Also acceptable: Ambient.

Unacceptable Conditions
Remarks

Posttransplant results will be compared to pretransplant recipient and donor genotypes, therefore, donor and recipient samples must be obtained and genotyped before the transplant event occurs.

Stability

Room temperature: 1 week; Refrigerated: 1 month; Frozen: Unacceptable

Reference Interval

Interpretive Data

Background Information: Chimerism, Donor
Indication:
Monitoring for bone marrow transplant patients; interval between bone marrow transplantation and testing is necessary for proper interpretation of results.
Methodology:
PCR followed by capillary electrophoresis. Specimens are analyzed using 15 autosomal markers (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, THO1, D13S317, D16S539, D2S1338, D19S433, vWa, TPOX, D18S51, D5S818 and FGA) and one gender marker (amelogenin).
Limitations: Diagnostic errors can occur due to rare sequence variations.

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Note

Hotline History

N/A

CPT Codes

See CPT codes under Chimerism, Recipient, Pretransplant (3005449)

Components

Component Test Code* Component Chart Name LOINC
3005463 Chimerism Donor, Specimen
3005464 Chimerism Donor, Recipient Name
3005465 Chimerism Donor,InformativeLoci
3005466 Chimerism Donor, Interpretation
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • Bone marrow DNA typing
  • Chimerism evaluation
  • Pre transplant evaluation
  • Transplant analysis
  • Transplant evaluation
Chimerism, Donor