Chimerism, Recipient, Pretransplant

3005449
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Informative

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Additional Technical Information

Ordering Recommendation

Use to assess recipient genotype and compare to donor genotype prior to allogeneic hematopoietic stem cell transplant. Pretransplant genotyping is required to enable posttransplant chimerism and engraftment monitoring. For posttransplant monitoring, refer to Chimerism, Posttransplant (3005454).

Mnemonic

STR_PRE

Methodology

Polymerase Chain Reaction (PCR)/Fragment Analysis

Performed

Sun-Sat

Reported

Varies

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation
Collect

Lavender (EDTA), pink (K2EDTA), or yellow (ACD solution A or B). OR bone marrow in lavender (EDTA). OR buccal brushes from recipient.

Specimen Preparation

Transport 2 mL whole blood (Min: 1 mL), OR 1 mL bone marrow (Min: 1 mL), OR 2 buccal brushes in a sterile, dry tube. (Min: 2 brushes)

Storage/Transport Temperature

Refrigerated. Also acceptable: Ambient.

Unacceptable Conditions
Remarks

Posttransplant results will be compared to pretransplant recipient and donor genotypes, therefore, donor and recipient specimens must be obtained and genotyped before the transplant event occurs. If transplant event occurred prior to specimen collection, dry buccal brushes (not bloody) are acceptable.

Stability

Room temperature: 1 week; Refrigerated: 1 month; Frozen: Unacceptable

Reference Interval

Interpretive Data

Refer to report.

Compliance Category

Laboratory Developed Test (LDT)

Note

Hotline History

View Hotline History

 

Hotline History

Date of Change
Test Name Change
Methodology
Performance/Reported Schedule
Specimen Requirements
Reference Interval
Interpretive Data
Note
CPT Code
Component Change
Other Interface Change
New Test
Inactive
08/15/2022
X
N/A

CPT Codes

81265

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Components

Component Test Code* Component Chart Name LOINC
3005450 Chimerism Recipient Pre, Specimen
3005451 Chimerism Recipient Pre,InformativeLoci
3005452 Chimerism Recipient Pre, Interpretation
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • Bone marrow DNA typing
  • Chimerism evaluation
  • Pre transplant evaluation
  • Transplant analysis
  • Transplant evaluation
Chimerism, Recipient, Pretransplant