Ordering Recommendation

Comprehensive panel for the evaluation of ANCA-associated vasculitis. For the preferred first-line panel, see Myeloperoxidase (MPO) Antibody and Serine Proteinase 3 (PR3) Antibody with Reflex to Anti-Neutrophil Cytoplasmic Antibody, IgG by IFA (3003746).

For evaluation of autoimmune liver disease, use in conjunction with Autoimmune Liver Disease Reflexive Panel (3002479).

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP standard transport tube. (Min: 0.5 mL)

Storage/Transport Temperature


Unacceptable Conditions

CSF, plasma, urine, or other body fluids. Contaminated, hemolyzed, or severely lipemic specimens.


After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 30 days (avoid repeated freeze/thaw cycles)


Semi-Quantitative Indirect Fluorescent Antibody (IFA)/Semi-Quantitative Multiplex Bead Assay




2-5 days

Reference Interval

Test Number
Reference Interval
  Myeloperoxidase (MPO) Ab, IgG 19 AU/mL or less
  Serine Proteinase 3 (PR3) Ab, IgG 19 AU/mL or less
  ANCA IFA Pattern None Detected
  ANCA IFA Titer Less than 1:20

Interpretive Data

Refer to report.

Myeloperoxidase (MPO) Antibody 19 AU/mL or less: Negative
20-25 AU/mL: Equivocal
26 AU/mL or greater: Positive
Serine Proteinase 3 (PR3) Antibody 19 AU/mL or less: Negative
20-25 AU/mL: Equivocal
26 AU/mL or greater: Positive

Compliance Category



Specimens are screened for ANCA, MPO, and PR3. ANCA IFA is simultaneously tested on ethanol- and formalin-fixed slides to allow differentiation of C- and P-ANCA patterns.

Hotline History


CPT Codes

83516 x2; 86036


Component Test Code* Component Chart Name LOINC
0050526 Myeloperoxidase (MPO) Ab, IgG 6969-0
0050527 Serine Proteinase 3 (PR3) Ab, IgG 6968-2
3003764 ANCA IFA Pattern 21419-7
3003765 ANCA IFA Titer 29967-7
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • (Perinuclear Antineutrophil Cytoplasmic Antibody
  • ANCA
  • ANCA Reflex
  • Anti-Neutrophil Cytoplasmic Ab Test
  • Anti-Neutrophil Cytoplasmic Antibodies Panel, IgG
  • Anticytoplasmic Antibody
  • Antineutrophil Cytoplasmic Antibodies (ACPA)
  • Autoantibodies to Proteinase 3
  • c-ANCA
  • Cytoplasmic Neutrophil Antibodies
  • MPO)
  • P-ANCA
  • pANCA
  • PR3
  • Proteinase 3
ANCA-Associated Vasculitis Profile (ANCA/MPO/PR3)