ANCA-Associated Vasculitis Profile (ANCA/MPO/PR3)
Ordering Recommendation
Comprehensive panel for the evaluation of ANCA-associated vasculitis. For the preferred first-line panel, see Myeloperoxidase (MPO) Antibody and Serine Proteinase 3 (PR3) Antibody with Reflex to Anti-Neutrophil Cytoplasmic Antibody, IgG by IFA (3003746).
For evaluation of autoimmune liver disease, use in conjunction with Autoimmune Liver Disease Reflexive Panel (3002479).
Methodology
Semi-Quantitative Indirect Fluorescent Antibody (IFA)/Semi-Quantitative Multiplex Bead Assay
Performed
Sun-Sat
Reported
2-5 days
New York DOH Approval Status
Specimen Required
Serum separator tube.
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.5 mL)
Refrigerated.
CSF, plasma, urine, or other body fluids. Contaminated, hemolyzed, or severely lipemic specimens.
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
Reference Interval
Test Number |
Components |
Reference Interval |
---|---|---|
Myeloperoxidase (MPO) Ab, IgG | 19 AU/mL or less | |
Serine Proteinase 3 (PR3) Ab, IgG | 19 AU/mL or less | |
ANCA IFA Pattern | None Detected | |
ANCA IFA Titer | Less than 1:20 |
Interpretive Data
Refer to report.
Component | Interpretation |
---|---|
Myeloperoxidase (MPO) Antibody | 19 AU/mL or less Negative 20-25 AU/mL Equivocal 26 AU/mL or greater Positive |
Serine Proteinase 3 (PR3) Antibody | 19 AU/mL or less Negative 20-25 AU/mL Equivocal 26 AU/mL or greater Positive |
FDA
Note
Specimens are screened for ANCA, MPO and PR3. ANCA IFA is simultaneously tested on ethanol- and formalin-fixed slides to allow differentiation of C- and P-ANCA patterns.
Hotline History
CPT Codes
83516 x2; 86036
Components
Component Test Code* | Component Chart Name | LOINC |
---|---|---|
0050526 | Myeloperoxidase (MPO) Ab, IgG | 6969-0 |
0050527 | Serine Proteinase 3 (PR3) Ab, IgG | 6968-2 |
3003764 | ANCA IFA Pattern | 21419-7 |
3003765 | ANCA IFA Titer | 29967-7 |
Aliases
- (Perinuclear Antineutrophil Cytoplasmic Antibody
- ANCA
- ANCA Reflex
- Anti-Neutrophil Cytoplasmic Ab Test
- Anti-Neutrophil Cytoplasmic Antibodies Panel, IgG
- Anticytoplasmic Antibody
- Antineutrophil Cytoplasmic Antibodies (ACPA)
- Autoantibodies to Proteinase 3
- c-ANCA
- Cytoplasmic Neutrophil Antibodies
- MPO)
- P-ANCA
- pANCA
- PR3
- Proteinase 3