Ordering Recommendation

Comprehensive panel for the evaluation of ANCA-associated vasculitis. For the preferred first-line panel, see Myeloperoxidase (MPO) Antibody and Serine Proteinase 3 (PR3) Antibody with Reflex to Anti-Neutrophil Cytoplasmic Antibody, IgG by IFA (3003746).

For evaluation of autoimmune liver disease, use in conjunction with Autoimmune Liver Disease Reflexive Panel (3002479).


Semi-Quantitative Indirect Fluorescent Antibody (IFA)/Semi-Quantitative Multiplex Bead Assay




2-5 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.5 mL)

Storage/Transport Temperature


Unacceptable Conditions

CSF, plasma, urine, or other body fluids. Contaminated, hemolyzed, or severely lipemic specimens.


After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Reference Interval

Test Number
Reference Interval
  Myeloperoxidase (MPO) Ab, IgG 19 AU/mL or less
  Serine Proteinase 3 (PR3) Ab, IgG 19 AU/mL or less
  ANCA IFA Pattern None Detected
  ANCA IFA Titer Less than 1:20

Interpretive Data

Refer to report.

Component Interpretation
Myeloperoxidase (MPO) Antibody 19 AU/mL or less  Negative
20-25 AU/mL  Equivocal
26 AU/mL or greater  Positive
Serine Proteinase 3 (PR3) Antibody 19 AU/mL or less  Negative
20-25 AU/mL  Equivocal
26 AU/mL or greater  Positive

Compliance Category



Specimens are screened for ANCA, MPO and PR3. ANCA IFA is simultaneously tested on ethanol- and formalin-fixed slides to allow differentiation of C- and P-ANCA patterns.

Hotline History


CPT Codes

83516 x2; 86036


Component Test Code* Component Chart Name LOINC
0050526 Myeloperoxidase (MPO) Ab, IgG 6969-0
0050527 Serine Proteinase 3 (PR3) Ab, IgG 6968-2
3003764 ANCA IFA Pattern 21419-7
3003765 ANCA IFA Titer 29967-7
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • (Perinuclear Antineutrophil Cytoplasmic Antibody
  • ANCA
  • ANCA Reflex
  • Anti-Neutrophil Cytoplasmic Ab Test
  • Anti-Neutrophil Cytoplasmic Antibodies Panel, IgG
  • Anticytoplasmic Antibody
  • Antineutrophil Cytoplasmic Antibodies (ACPA)
  • Autoantibodies to Proteinase 3
  • c-ANCA
  • Cytoplasmic Neutrophil Antibodies
  • MPO)
  • P-ANCA
  • pANCA
  • PR3
  • Proteinase 3
ANCA-Associated Vasculitis Profile (ANCA/MPO/PR3)