Recommended initial screening panel when autoimmune liver disease is strongly suspected. If negative, less frequently occurring autoantibody tests maybe ordered based on patient’s clinical presentation and history.
Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Semi-Quantitative Indirect Fluorescent Antibody
New York DOH Approval Status
Serum Separator Tube (SST).
Separate from cells ASAP or within 2 hours of collection. Transfer 1.5 mL serum to an ARUP Standard Transport Tube. (Min: 1.0 mL)
Non-serum, heat-inactivated, contaminated, grossly icteric, severely lipemic, grossly hemolyzed specimens or inclusion of fibrin clot.
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
|Mitochondrial (M2) Antibody, IgG||24.9 Units or less|
|Soluble Liver Antigen Antibody, IgG||24.9 U or less|
|Liver-Kid Microsome-1 Ab, IgG by ELISA||24.9 U or less|
|F-Actin (Smooth Muscle) Ab, IgG by ELISA||19 Units or less|
|Antinuclear Antibody (ANA), HEp-2, IgG||Less than 1:80|
|Mitochondrial M2 Antibody, IgG (ELISA)||20.0 Units or less Negative
20.1-24.9 Units Equivocal
25.0 Units or greater Positive
|Soluble Liver Antigen Antibody, IgG||0.0-20.0 U Negative
20.1-24.9 U Equivocal
Greater than or equal to 25.0 U Positive
|Liver-Kidney Microsome - 1 Antibody, IgG||0.0-20.0 U Negative
20.1-24.9 U Equivocal
25.0 U or greater Positive
|F-Actin (Smooth Muscle) Ab, IgG by ELISA||19 Units or less Negative
20 - 30 Units Weak Positive-Suggest repeat testing in two to three weeks with fresh specimen.
31 Units or greater Positive-Suggestive of autoimmune hepatitis type 1 or chronic active hepatitis.
If F-Actin, IgG by ELISA is 20 Units or greater, then Smooth Muscle Antibody (SMA), IgG by IFA titer will be added. Additional charges apply.
ANA identified by indirect fluorescence assay (IFA) using HEp-2 substrate and IgG-specific conjugate at a screening dilution of 1:80. Positive nuclear patterns reported include homogeneous, speckled, centromere, nucleolar, or nuclear dots. Positive cytoplasmic patterns reported include reticular/AMA, discrete/GW body-like, polar/golgi-like, rods and rings, or cytoplasmic speckled patterns. All positive results are reported with endpoint titers at no additional charge.
86381; 83516; 86376; 86015; 86039; if reflexed, add 86256
|Component Test Code*||Component Chart Name||LOINC|
|0050065||Mitochondrial (M2) Antibody, IgG||14251-3|
|0055235||Soluble Liver Antigen Antibody, IgG||38420-6|
|0055241||Liver-Kid Microsome-1 Ab, IgG by ELISA||47318-1|
|0055248||F-Actin (Smooth Muscle) Ab, IgG by ELISA||44706-0|
|3000090||Antinuclear Antibody (ANA), HEp-2, IgG||21424-7|
|3000096||ANA Interpretive Comment||49311-4|