Ordering Recommendation
Recommended initial screening panel when autoimmune liver disease is strongly suspected. If negative, less frequently occurring autoantibody tests maybe ordered based on patient’s clinical presentation and history.
Mnemonic
Methodology
Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Semi-Quantitative Indirect Fluorescent Antibody
Performed
Sun-Sat
Reported
1-8 days
New York DOH Approval Status
Specimen Required
Serum Separator Tube (SST).
Separate from cells ASAP or within 2 hours of collection. Transfer 0.5 mL serum to an ARUP Standard Transport Tube. (Min: 0.3 mL)
Refrigerated.
Non-serum, heat-inactivated, contaminated, grossly icteric, severely lipemic, grossly hemolyzed specimens or inclusion of fibrin clot.
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
Reference Interval
Test Number |
Components |
Reference Interval |
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---|---|---|---|---|---|---|---|---|---|---|---|
0050065 | Mitochondrial M2 Antibody, IgG (ELISA) |
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0051174 | F-Actin (Smooth Muscle) Antibody, IgG by ELISA with Reflex to Smooth Muscle Antibody, IgG Titer |
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0055235 | Soluble Liver Antigen Antibody, IgG |
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0055241 | Liver-Kidney Microsome - 1 Antibody, IgG |
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3000082 | Antinuclear Antibody (ANA) with HEp-2 Substrate, IgG by IFA | Less than 1:80 |
Interpretive Data
Refer to report.
FDA
Note
If F-Actin, IgG by ELISA is 20 Units or greater, then Smooth Muscle Antibody (SMA), IgG by IFA titer will be added. Additional charges apply.
ANA identified by indirect fluorescence assay (IFA) using HEp-2 substrate and IgG-specific conjugate at a screening dilution of 1:80. Positive nuclear patterns reported include homogeneous, speckled, centromere, nucleolar, or nuclear dots. Positive cytoplasmic patterns reported include reticular/AMA, discrete/GW body-like, polar/golgi-like, rods and rings, or cytoplasmic speckled patterns. All positive results are reported with endpoint titers at no additional charge.
Hotline History
Hotline History
CPT Codes
86381; 83516; 86376; 86015; 86039; if reflexed, add 86256
Components
Component Test Code* | Component Chart Name | LOINC |
---|---|---|
0050065 | Mitochondrial (M2) Antibody, IgG | 14251-3 |
0055235 | Soluble Liver Antigen Antibody, IgG | 38420-6 |
0055241 | Liver-Kid Microsome-1 Ab, IgG by ELISA | 47318-1 |
0055248 | F-Actin (Smooth Muscle) Ab, IgG by ELISA | 44706-0 |
3000090 | Antinuclear Antibody (ANA), HEp-2, IgG | 21424-7 |
3000096 | ANA Interpretive Comment | 49311-4 |