Ordering Recommendation

First-line serologic screening test for suspected Lyme disease. Positive or equivocal results must be confirmed by immunoblot.

Mnemonic
LYME PEP
Methodology

Semi-Quantitative Enzyme-Linked Immunosorbent Assay

Performed

Mon-Fri

Reported

1-4 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Serum separator tube.

Specimen Preparation

Remove serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.15 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Plasma, CSF. Contaminated, heat-inactivated, hemolyzed, icteric, or lipemic specimens.

Remarks
Stability

After separation from cells: Ambient: 48 hours; Refrigerated: 10 days; Frozen: 1 month

Reference Interval
Reference Interval
0.90 IV or less Negative - VlsE1 and pepC10 antibodies to B. burgdorferi not detected.
0.91-1.09 IV Equivocal - Repeat testing in 10-14 days may be helpful.
1.10 IV or greater Positive - VlsE1 and pepC10 antibodies to B. burgdorferi detected.

Interpretive Data



Compliance Category

FDA

Note
Hotline History
N/A
CPT Codes

86618

Components
Component Test Code* Component Chart Name LOINC
3003258 B. burgdorferi VlsE1/pepC10 Abs, ELISA 5060-9
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Lyme Disease
  • Lyme Disease C6 Peptide Ab, ELISA
  • VlsE Lipoprotein
  • VlsE1/pepC10 Assay
Borrelia burgdorferi VlsE1/pepC10 Antibodies, Total by ELISA