Ordering Recommendation

Preferred reflex test to detect Lyme disease in individuals with >4 weeks of clinical symptoms or exposure to tick. Positive/equivocal screen confirmed by immunoblot.

Mnemonic

LPEP CHRON

Methodology

Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Qualitative Immunoblot

Performed

Mon-Fri

Reported

1-4 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation
Collect

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 2 mL serum to an ARUP Standard Transport Tube. (Min: 0.15 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Plasma, CSF. Contaminated, heat-inactivated, hemolyzed, icteric, or lipemic specimens.

Remarks
Stability

After separation from cells: Ambient: 48 hours; Refrigerated: 10 days; Frozen: 1 month

Reference Interval

Test Number
Components
Reference Interval
  B. burgdorferi VlsE1/pepC10 Abs, ELISA 0.90 IV or less

Interpretive Data




Component Interpretation
B. burgdorferi VlsE1/pepC10 Abs, ELISA 0.90 IV or less   Negative - VlsE1 and pepC10 antibodies to B. burgdorferi not detected.
0.91-1.09 IV   Equivocal - Repeat testing in 10-14 days may be helpful.
1.10 IV or greater   Positive - VlsE1 and pepC10 antibodies to B. burgdorferi detected.

Compliance Category

FDA

Note

If VlsE1/pepC10 antibodies by ELISA is 0.91 IV or greater, then IgG Immunoblot will be added. Additional charges apply.

Hotline History

N/A

CPT Codes

86618; if reflexed, add 86617

Components

Component Test Code* Component Chart Name LOINC
3003258 B. burgdorferi VlsE1/pepC10 Abs, ELISA
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • Lyme Disease VlsE1/pepC10 Ab, ELISA Reflex to IgG WB
  • Lyme Disease
  • Lyme Disease C6 Peptide Ab Reflex Panel
  • VlsE Lipoprotein, VlsE1/pepC10 Assay
Borrelia burgdorferi VlsE1/pepC10 Antibodies, Total by ELISA with Reflex to IgG by Immunoblot