Borrelia burgdorferi VlsE1/pepC10 Antibodies, Total by ELISA with Reflex to IgG by Immunoblot
Ordering Recommendation
Preferred reflex test to detect Lyme disease in individuals with >4 weeks of clinical symptoms or exposure to tick. Positive/equivocal screen confirmed by immunoblot.
Mnemonic
Methodology
Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Qualitative Immunoblot
Performed
Mon-Fri
Reported
1-4 days
New York DOH Approval Status
Specimen Required
Serum separator tube.
Separate serum from cells ASAP or within 2 hours of collection. Transfer 2 mL serum to an ARUP Standard Transport Tube. (Min: 0.15 mL)
Refrigerated.
Plasma, CSF. Contaminated, heat-inactivated, hemolyzed, icteric, or lipemic specimens.
After separation from cells: Ambient: 48 hours; Refrigerated: 10 days; Frozen: 1 month
Reference Interval
Test Number |
Components |
Reference Interval |
---|---|---|
B. burgdorferi VlsE1/pepC10 Abs, ELISA | 0.90 IV or less |
Interpretive Data
Component | Interpretation |
---|---|
B. burgdorferi VlsE1/pepC10 Abs, ELISA | 0.90 IV or less Negative - VlsE1 and pepC10 antibodies to B. burgdorferi not detected. 0.91-1.09 IV Equivocal - Repeat testing in 10-14 days may be helpful. 1.10 IV or greater Positive - VlsE1 and pepC10 antibodies to B. burgdorferi detected. |
FDA
Note
If VlsE1/pepC10 antibodies by ELISA is 0.91 IV or greater, then IgG Immunoblot will be added. Additional charges apply.
Hotline History
CPT Codes
86618; if reflexed, add 86617
Components
Component Test Code* | Component Chart Name | LOINC |
---|---|---|
3003258 | B. burgdorferi VlsE1/pepC10 Abs, ELISA | 100711-1 |
Aliases
- Lyme Disease VlsE1/pepC10 Ab, ELISA Reflex to IgG WB
- Lyme Disease
- Lyme Disease C6 Peptide Ab Reflex Panel
- VlsE Lipoprotein, VlsE1/pepC10 Assay