Ordering Recommendation

Reflex test to diagnose Lyme disease in symptomatic individuals. For use >4 week after symptom onset. Reflex pattern follows the standard two-tier testing (STTT) approach; a positive or equivocal screen is confirmed by immunoblot. 
The preferred reflex test is Borrelia burgdorferi VlsE1/pepC10 Antibodies, Total by ELISA With Reflex to IgM and IgG by ELISA (Modified Two-Tier Testing) (3006053).

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 2 mL serum to an ARUP Standard Transport Tube. (Min: 0.15 mL)

Storage/Transport Temperature


Unacceptable Conditions

Plasma, CSF. Contaminated, heat-inactivated, hemolyzed, icteric, or lipemic specimens.


After separation from cells: Ambient: 48 hours; Refrigerated: 10 days; Frozen: 1 month


Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Qualitative Immunoblot




1-4 days

Reference Interval

Test Number
Reference Interval
  B. burgdorferi VlsE1/pepC10 Abs, ELISA 0.90 IV or less

Interpretive Data

Component Interpretation
B. burgdorferi VlsE1/pepC10 Abs, ELISA 0.90 IV or less   Negative - VlsE1 and pepC10 antibodies to B. burgdorferi not detected.
0.91-1.09 IV   Equivocal - Repeat testing in 10-14 days may be helpful.
1.10 IV or greater   Positive - VlsE1 and pepC10 antibodies to B. burgdorferi detected.

Compliance Category



If VlsE1/pepC10 antibodies by ELISA is 0.91 IV or greater, then IgG Immunoblot will be added. Additional charges apply.

Hotline History


CPT Codes

86618; if reflexed, add 86617


Component Test Code* Component Chart Name LOINC
3003258 B. burgdorferi VlsE1/pepC10 Abs, ELISA 100711-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • Lyme Disease VlsE1/pepC10 Ab, ELISA Reflex to IgG WB
  • Lyme Disease
  • Lyme Disease C6 Peptide Ab Reflex Panel
  • VlsE Lipoprotein, VlsE1/pepC10 Assay
Borrelia burgdorferi VlsE1/pepC10 Antibodies, Total by ELISA with Reflex to IgG by Immunoblot