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Borrelia burgdorferi VlsE1/pepC10 Antibodies, Total by ELISA With Reflex to IgM and IgG by ELISA (Modified Two-Tier Testing)

LYME MTTT
Example Reports
Negative
Positive

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Additional Technical Information

Ordering Recommendation

Preferred reflex test to diagnose Lyme disease in symptomatic individuals. Reflex pattern follows the modified two-tier testing (MTTT) approach; a positive or equivocal screen is confirmed by immunoassay.

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation
Collect

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.15 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

CSF or plasma. Contaminated, heat-inactivated, hemolyzed, or severely lipemic specimens.

Remarks
Stability

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 month (avoid repeated freeze/thaw cycles)

Methodology

Semiquantitative Enzyme-Linked Immunosorbent Assay

Performed

Mon-Fri

Reported

1-4 days

Reference Interval

Test Number
 Components
 Reference Interval
  B. burgdorferi VlsE1/pepC10 Abs, ELISA 0.90 IV or less

Interpretive Data

Refer to report.


Component Interpretation
B. Burgdorferi VlsE1/pepC10 Abs, ELISA 0.90 IV or less; Negative - VlsE1 and pepC10 antibodies to B. burgdorferi not detected.
0.91-1.09 IV; Equivocal - Repeat testing in 10-14 days may be helpful.
1.10 IV or greater; Positive - VlsE1 and pepC10 antibodies to B. burgdorferi detected.

Compliance Category

FDA

Note

If VlsE1/pepC10 antibodies by ELISA is 0.91 IV or greater, then IgM and IgG by ELISA will be added. Additional charges apply.

Hotline History

N/A

CPT Codes

86618; if reflexed, add 86618 x2

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Components

Component Test Code* Component Chart Name LOINC
3003258 B. burgdorferi VlsE1/pepC10 Abs, ELISA 100711-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • Lyme disease C6 peptide Ab reflex panel, Modified 2-tier testing
  • VlsE lipoprotein, VlsE1/pepC10 assay
  • MTTT
  • Lyme disease
  • Lyme disease VlsE1/pepC10 Total Ab, ELISA reflex to IgM and IgM ELISAs
Borrelia burgdorferi VlsE1/pepC10 Antibodies, Total by ELISA With Reflex to IgM and IgG by ELISA (Modified Two-Tier Testing)