Borrelia burgdorferi VlsE1/pepC10 Antibodies, Total by ELISA With Reflex to IgM and IgG by ELISA (Modified Two-Tier Testing)

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Example Reports
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Positive

Ordering Recommendation

Preferred reflex test to diagnose Lyme disease in symptomatic individuals. Reflex pattern follows the modified two-tier testing (MTTT) approach; a positive or equivocal screen is confirmed by immunoassay.

Mnemonic

LYME MTTT

Methodology

Semiquantitative Enzyme-Linked Immunosorbent Assay

Performed

Mon-Fri

Reported

1-4 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation
Collect

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.15 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

CSF or plasma. Contaminated, heat-inactivated, hemolyzed, or severely lipemic specimens.

Remarks
Stability

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 month (avoid repeated freeze/thaw cycles)

Reference Interval

Test Number
 Components
 Reference Interval
  B. burgdorferi VlsE1/pepC10 Abs, ELISA 0.90 IV or less

Interpretive Data

Refer to report.


Component Interpretation
B. Burgdorferi VlsE1/pepC10 Abs, ELISA 0.90 IV or less; Negative - VlsE1 and pepC10 antibodies to B. burgdorferi not detected.
0.91-1.09 IV; Equivocal - Repeat testing in 10-14 days may be helpful.
1.10 IV or greater; Positive - VlsE1 and pepC10 antibodies to B. burgdorferi detected.

Compliance Category

FDA

Note

If VlsE1/pepC10 antibodies by ELISA is 0.91 IV or greater, then IgM and IgG by ELISA will be added. Additional charges apply.

Hotline History

View Hotline History

 

Hotline History

Date of Change
Test Name Change
Methodology
Performance/Reported Schedule
Specimen Requirements
Reference Interval
Interpretive Data
Note
CPT Code
Component Change
Other Interface Change
New Test
Inactive
03/27/2023
X
N/A

CPT Codes

86618; if reflexed, add 86618 x2

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Components

Component Test Code* Component Chart Name LOINC
3003258 B. burgdorferi VlsE1/pepC10 Abs, ELISA 100711-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • Lyme disease
  • Lyme disease C6 peptide Ab reflex panel, Modified 2-tier testing
  • Lyme disease VlsE1/pepC10 Total Ab, ELISA reflex to IgM and IgM ELISAs
  • MTTT
  • VlsE lipoprotein, VlsE1/pepC10 assay
Borrelia burgdorferi VlsE1/pepC10 Antibodies, Total by ELISA With Reflex to IgM and IgG by ELISA (Modified Two-Tier Testing)