Ordering Recommendation

Not recommended for the diagnosis of fungal infection. Includes testing by complement fixation for Aspergillus, Coccidioides, and Histoplasma and testing by immunoassay for Blastomyces. Refer to individual fungus-specific testing according to patient exposure: Coccidioides Antibodies Panel, Serum (0050588); Aspergillus Antibodies by Complement Fixation and Immunodiffusion, Serum (0050101); Histoplasma Antibodies by Complement Fixation and Immunodiffusion, Serum (0050627); and Blastomyces dermatitidis Antibodies by Immunoassay with Reflex to Immunodiffusion, Serum (3000236).

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation

Serum separator tube.

Specimen Preparation

Separate from cells ASAP or within 2 hours of collection. Transfer 2 mL serum to an ARUP standard transport tube. (Min: 1.2 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" or "convalescent."

Storage/Transport Temperature


Unacceptable Conditions

Contaminated, hemolyzed, or severely lipemic specimens.


After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)


Semi-Quantitative Complement Fixation/Semi-Quantitative Enzyme-Linked Immunosorbent Assay (ELISA)/Qualitative Immunodiffusion




2-6 days

Reference Interval

Test Number
Reference Interval
  Aspergillus Antibodies by CF Less than 1:8
  Coccidioides Antibody by CF Less than 1:2
  Histoplasma Mycelia Antibodies by CF Less than 1:8
  Histoplasma Yeast Antibodies by CF Less than 1:8
  Blastomyces Antibodies EIA, SER 0.9 IV or less

Interpretive Data

Refer to report.

Compliance Category



This test detects antibodies to Aspergillus, Coccidioides, and Histoplasma by complement fixation and Blastomyces by immunoassay.
If Blastomyces antibodies are equivocal or positive by immunoassay, then Blastomyces dermatitidis Antibodies by Immunodiffusion will be added. Additional charges apply.

Hotline History


CPT Codes

86606; 86612; 86635; 86698 x2; if reflexed, add 86612


Component Test Code* Component Chart Name LOINC
0050100 Aspergillus Antibodies by CF 5053-4
0050170 Coccidioides Antibody by CF 33380-7
0050330 Histoplasma Mycelia Antibodies by CF 20573-2
0050335 Histoplasma Yeast Antibodies by CF 20574-0
3000237 Blastomyces Antibodies EIA, SER 16471-5
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • Aspergillus
  • Blastomyces
  • Coccidiodes
  • Histoplasma
Fungal Antibodies with Reflex to Blastomyces dermatitidis Antibodies by Immunodiffusion, Serum