Ordering Recommendation

Aids in the diagnosis of histoplasmosis. Testing in conjunction with Histoplasma Antigen Quantitative by EIA, Serum (0092522) and Histoplasma Galactomannan Antigen Quantitative by EIA, Urine (2009418) is recommended.

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 2 mL serum to an ARUP standard transport tube. (Min: 0.8 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" or "convalescent."

Storage/Transport Temperature


Unacceptable Conditions

Contaminated, hemolyzed, or severely lipemic specimens.


After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)


Semi-Quantitative Complement Fixation/Qualitative Immunodiffusion




3-6 days

Reference Interval

Test Number
Reference Interval
  Histoplasma Antibodies by ID Not detected.
  Histoplasma Mycelia Antibodies by CF Less than 1:8
  Histoplasma Yeast Antibodies by CF Less than 1:8

Interpretive Data

Refer to report.

Compliance Category



The immunodiffusion component of this test detects total antibodies against the H and M antigens of Histoplasma capsulatum. The complement fixation component of this test detects total antibodies to mycelial and yeast antigens of Histoplasma.

Hotline History


CPT Codes

86698 x3


Component Test Code* Component Chart Name LOINC
0050174 Histoplasma Antibodies by ID 5218-3
0050330 Histoplasma Mycelia Antibodies by CF 20573-2
0050335 Histoplasma Yeast Antibodies by CF 20574-0
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • H antigen
  • Histoplasma capsulatum
  • Histoplasmosis
  • M antigen
  • Precipitin
Histoplasma Antibodies by Complement Fixation and Immunodiffusion