Ordering Recommendation

Laboratory reference method for determining free and total testosterone in men. Total or free testosterone is generally adequate for most evaluations of suspected hypogonadism.

Mnemonic

TE F&T ED

Methodology

Quantitative Equilibrium Dialysis/High Performance Liquid Chromatography-Tandem Mass Spectrometry

Performed

Sun, Wed-Sat

Reported

1-6 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation

Collect between 6-10 a.m.

Collect

Serum separator tube or green (sodium or lithium heparin).

Specimen Preparation

Separate serum or plasma from cells ASAP or within 2 hours of collection. Transport 1 mL serum or plasma. (Min: 0.4 mL)

Storage/Transport Temperature

Frozen.

Unacceptable Conditions

EDTA plasma.

Remarks
Stability

After separation from cells: Ambient: 24 hours; Refrigerated: 1 week; Frozen: 2 months

Reference Interval

Male Free Testosterone, pg/mL
18 years and older: 47-244 pg/mL

Male Total Testosterone, ng/dL
18-39 years: 300-1080 ng/dL
40-59 years: 300-890 ng/dL
60 years and older: 300-720 ng/dL

Interpretive Data

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Laboratory Developed Test (LDT)

Note

Hotline History

N/A

CPT Codes

84402; 84403

Components

Component Test Code* Component Chart Name LOINC
2003247 Testos Free Adult Male ED/LC-MS/MS 2991-8
2004245 Testosterone Total, LC-MS/MS, Males 2986-8
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • Direct dialysis (Testosterone Free & Total by ED/LC-MS/MS (Free) and LC-MS/MS (Total), Adult Males)
  • Total and Free (Testosterone Free & Total by ED/LC-MS/MS (Free) and LC-MS/MS (Total), Adult Males)
Testosterone Free and Total by ED/LC-MS/MS (Free) and LC-MS/MS (Total), Adult Males