Ordering Recommendation

Laboratory reference method for determining free and total testosterone in men. Total or free testosterone is generally adequate for most evaluations of suspected hypogonadism.

Mnemonic
TE F&T ED
Methodology

Quantitative Equilibrium Dialysis/High Performance Liquid Chromatography-Tandem Mass Spectrometry

Performed

Sun, Wed-Sat

Reported

1-6 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation

Collect between 6-10 a.m.

Collect

Serum separator tube or green (sodium or lithium heparin).

Specimen Preparation

Separate serum or plasma from cells ASAP or within 2 hours of collection. Transport 1 mL serum or plasma. (Min: 0.4 mL)

Storage/Transport Temperature

Frozen.

Unacceptable Conditions

EDTA plasma.

Remarks
Stability

After separation from cells: Ambient: 24 hours; Refrigerated: 1 week; Frozen: 2 months

Reference Interval
Male Free Testosterone, pg/mL
18 years and older: 47-244 pg/mL

Male Total Testosterone, ng/dL
18-39 years: 300-1080 ng/dL
40-59 years: 300-890 ng/dL
60 years and older: 300-720 ng/dL

Interpretive Data



Compliance Category

Laboratory Developed Test (LDT)

Note
Hotline History
N/A
CPT Codes

84402; 84403

Components
Component Test Code* Component Chart Name LOINC
2003247 Testos Free Adult Male ED/LC-MS/MS 2991-8
2004245 Testosterone Total, LC-MS/MS, Males 2986-8
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Direct dialysis (Testosterone Free & Total by ED/LC-MS/MS (Free) and LC-MS/MS (Total), Adult Males)
  • Total and Free (Testosterone Free & Total by ED/LC-MS/MS (Free) and LC-MS/MS (Total), Adult Males)
Testosterone Free and Total by ED/LC-MS/MS (Free) and LC-MS/MS (Total), Adult Males