Testosterone, Free and Total, Includes Sex Hormone-Binding Globulin (Adult Females, Children, or Individuals on Testosterone-Suppressing Hormone Therapy)
Provides a calculated value for free testosterone concentration using total testosterone measured by mass spectrometry. May be used to evaluate hyperandrogenism in children and cisgender females, monitor testosterone-suppressing hormone therapies (eg, antiandrogens or estrogens), evaluate testosterone status in individuals with protein-binding abnormalities, or evaluate hypogonadism in cisgender males.
Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry/Electrochemiluminescent Immunoassay
The concentration of free testosterone is derived from a mathematical expression based on the constant for the binding of testosterone to sex hormone binding globulin.
New York DOH Approval Status
Collect between 6-10 a.m.
Serum separator tube or green (sodium or lithium heparin).
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.8 mL)
After separation from cells: Ambient: 24 hours; Refrigerated: 1 week; Frozen: 6 months
|Testosterone by Mass Spec||
|Testosterone, Free by Mass Spec||
|Sex Hormone Binding Globulin||
Free testosterone concentration is calculated using total testosterone (measured by mass spectrometry) and the binding constant of testosterone and sex hormone-binding globulin (SHBG).
For individuals on testosterone-suppressing hormone therapies (e.g., antiandrogens or estrogens), refer to cisgender female reference intervals. For a complete set of all established reference intervals, refer to ltd.aruplab.com/Tests/Pub/0081056.
This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the U.S. Food and Drug Administration. This test was performed in a CLIA-certified laboratory and is intended for clinical purposes.
Laboratory Developed Test (LDT)
Please refer to individual components for stability of sample for this test.
84402; 84403; 84270
|Component Test Code*||Component Chart Name||LOINC|
|0081058||Testosterone by Mass Spec||2986-8|
|0081096||Testosterone, Free by Mass Spec||49042-5|
|0099375||Sex Hormone Binding Globulin||13967-5|
- Testosterone Free