Testosterone, Free and Total, Includes Sex Hormone-Binding Globulin (Adult Females, Children, or Individuals on Testosterone-Suppressing Hormone Therapy)
Provides a calculated value for free testosterone concentration using total testosterone measured by mass spectrometry. May be used to evaluate hyperandrogenism in children and cisgender females, monitor testosterone-suppressing hormone therapies (eg, antiandrogens or estrogens), evaluate testosterone status in individuals with protein-binding abnormalities, or evaluate hypogonadism in cisgender males.
To compare this test to other testosterone tests, refer to the ARUP Testosterone Tests Comparison table.
New York DOH Approval Status
Collect between 6-10 a.m.
Serum separator tube or green (sodium or lithium heparin).
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum or plasma to an ARUP standard transport tube. (Min: 0.8 mL)
After separation from cells: Ambient: 24 hours; Refrigerated: 1 week; Frozen: 6 months
Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry/Electrochemiluminescent Immunoassay/Calculation
|Testosterone by Mass Spec||
|Testosterone, Free by Mass Spec||
|Sex Hormone Binding Globulin||
Free testosterone concentration is calculated using total testosterone (measured by mass spectrometry) and the binding constant of testosterone and sex hormone-binding globulin (SHBG).
For individuals on testosterone-suppressing hormone therapies (e.g., antiandrogens or estrogens), refer to cisgender female reference intervals. For a complete set of all established reference intervals, refer to ltd.aruplab.com/Tests/Pub/0081056.
Laboratory Developed Test (LDT)
Please refer to individual components for stability of sample for this test.
The concentration of free testosterone is derived from a mathematical expression based on the constant for the binding of testosterone to sex hormone binding globulin.
84402; 84403; 84270
|Component Test Code*||Component Chart Name||LOINC|
|0081058||Testosterone by Mass Spec||2986-8|
|0081096||Testosterone, Free by Mass Spec||49042-5|
|0099375||Sex Hormone Binding Globulin||13967-5|
- Testosterone Free