Ordering Recommendation

Aids in the evaluation of suspected hypogonadism in men with a total testosterone level at the lower limit of the normal range. Not recommended for use in women and children.




Quantitative Electrochemiluminescent Immunoassay
The concentration of free testosterone is derived from a mathematical expression based on the constant for the binding of testosterone to sex hormone binding globulin.




Within 24 hours

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation

Collect specimen between 6-10 a.m.


Serum separator tube or green (lithium heparin).

Specimen Preparation

Separate serum or plasma from cells immediately after collection. Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.6 mL)

Storage/Transport Temperature


Unacceptable Conditions

EDTA plasma.


After separation from cells: Ambient: 48 hours; Refrigerated: 1 week; Frozen: 2 months

Reference Interval

Test Number
Reference Interval
  Testosterone, Percentage Free 18 years and older:  1.6-2.9%
0099375 Sex Hormone Binding Globulin
Age Male Female
1-30 days
31-364 days
1-3 years
4-6 years
7-9 years
10-12 years
13-15 years
16-17 years
18 years and older
13-85 nmol/L
70-250 nmol/L
50-180 nmol/L
45-175 nmol/L
28-190 nmol/L
23-160 nmol/L
13-140 nmol/L
10-60 nmol/L
11-80 nmol/L
14-60 nmol/L
60-215 nmol/L
60-190 nmol/L
55-170 nmol/L
35-170 nmol/L
17-155 nmol/L
11-120 nmol/L
19-145 nmol/L
30-135 nmol/L
Tanner Stage I
Tanner Stage II
Tanner Stage III
Tanner Stage IV
Tanner Stage V
26-186 nmol/L
22-169 nmol/L
13-104 nmol/L
11-60 nmol/L
11-71 nmol/L
30-173 nmol/L
16-127 nmol/L
12-98 nmol/L
14-151 nmol/L
23-165 nmol/L

0070111 Testosterone Free, Adult Male Male:
14-15 years:  3-138 pg/mL
16-17 years:  38-173 pg/mL
18 years and older: 47-244 pg/mL

Tanner Stage IV:  35-169 pg/mL
Tanner Stage V:  41-239 pg/mL
0070130 Testosterone, Adult Male Male:
14-15 years: 33-585 ng/dL
16-17 years: 185-886 ng/dL
18-39 years: 300-1080 ng/dL
40-59 years: 300-890 ng/dL
60 years and older: 300-720 ng/dL

Tanner Stage IV: 165-854 ng/dL
Tanner Stage V: 194-783 ng/dL

Interpretive Data

The concentration of Free Testosterone is derived from a mathematical expression based on the constant for the binding of testosterone to Sex Hormone Binding Globulin (SHBG).

Compliance Category



Please refer to individual components for stability of sample for this test.  This test is not recommended for females or children.   For testing on females or children refer to Testosterone, Free & Total, (Includes Sex Hormone Binding Globulin), Females and Children (0081056).

Hotline History


CPT Codes

84402; 84403; 84270


Component Test Code* Component Chart Name LOINC
0070113 Testosterone, Free Calculation 2991-8
0070114 Testosterone, Percentage Free 15432-8
0070130 Testosterone, Adult Male 2986-8
0099375 Sex Hormone Binding Globulin 13967-5
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • Free Testosterone
  • Male testosterone testing
  • SHBG
  • Total Testosterone
Testosterone, Free and Total (Includes Sex Hormone Binding Globulin), Adult Male