Ordering Recommendation

Monitor engraftment of donor cells post allogenic stem cell transplantation. Each cell sort must have a unique ARUP accession number.




Polymerase Chain Reaction/Fragment Analysis




5-9 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation

Lavender (EDTA), pink (K2EDTA), or yellow (ACD Solution A or B). OR bone marrow in lavender (EDTA).

Specimen Preparation

Transport 3 mL whole blood. (Min: 3 mL) OR 1 mL bone marrow. Ship overnight. If cell sorting is required, specimens should be received within 24 hours of collection for optimal isolation of the requested cell line(s). Increase the amount of blood/bone marrow submitted based on the number of cell lines requested and for patients with low cell counts.

Storage/Transport Temperature


Unacceptable Conditions

Clotted or hemolyzed specimens.


Post-transplantation genotypes will be compared to pre-transplant recipient and donor genotypes. Therefore, donor and recipient specimens must be obtained and genotyped before the transplant event occurs. Please provide the results and date of the patient's most recent WBC and differential counts. When submitting bone marrow specimens for cell sorting, please provide information regarding the general cellularity of the patient's bone marrow. See Cell Isolation Request for Chimerism, Post-Transplant, Sorted Cells.


Ambient: 48 hours; Refrigerated: 48 hours; Frozen: Unacceptable

Reference Interval

Type Donor - Donor cells only.
Type Recipient - Recipient cells only.
Mixed - Donor and Recipient cells present.  Semi-quantitative results of percentage of donor and recipient cells will be reported.

Interpretive Data

Background Information for Chimerism, Post-Transplant, Sorted Cells:
Monitoring for bone marrow transplant patients; correlation with clinical status and consideration of the interval between bone marrow transplantation and testing is necessary for proper interpretation of results.
Methodology: PCR followed by capillary electrophoresis. Specimens are analyzed using 15 autosomal markers (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, THO1, D13S317, D16S539, D2S1338, D19S433, vWa, TPOX, D18S51, D5S818, and FGA) and one gender marker (amelogenin).
Kit Used:
AmpFLSTR Identifiler® PCR Amplification Kit, Applied Biosystems.
Limit of Detection:
2 percent of minor cell population.

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Laboratory Developed Test (LDT)


If requesting multiple cell lines, additional charges apply and vary based on the cell line requested (BMT Cell Isolation or BMT Chimerism Sort). Chimerism, Post-Transplant, Sorted Cells will be billed as separate tests for each cell line requested.

Hotline History


CPT Codes

81268; If cell sorting is performed, add 88184 or 88184; 88185


Component Test Code* Component Chart Name LOINC
0051670 Chimerism,Post-Cell Sort, Interpretation 50398-7
2001380 Chimerism,Post-Cell Sort, Specimen 66746-9
2002034 Chimerism,Post-Cell Sort,Margin of Error
2002035 Chimerism,Post-Cell Sort, Percent Recip
2002036 Chimerism,Post-Cell Sort, Percent Donor
2002037 Chimerism,Post-Cell Sort,InformativeLoci
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • Bone Marrow DNA typing
  • Bone Marrow DNA Typing Transplant Eval., Post-Transplant
  • Bone Marrow Post-Trans Eval
  • Bone Marrow Transplantation; Chimerism Evaluation
  • Engraftment Monitoring
  • Transplant Analysis
  • Transplant Evaluation
Chimerism, Post-Transplant, Sorted Cells (Extended TAT as of 11/20/20-no referral available)