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Chimerism, Post-Transplant, Sorted Cells
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Monitor engraftment of donor cells post allogenic stem cell transplantation. Each cell sort must have a unique ARUP accession number.
Polymerase Chain Reaction/Fragment Analysis
Sun-Sat
5-9 days
Lavender (EDTA), pink (K2EDTA), or yellow (ACD Solution A or B). OR bone marrow in lavender (EDTA).
Transport 3 mL whole blood. (Min: 3 mL) OR 1 mL bone marrow. Ship overnight. If cell sorting is required, specimens should be received within 24 hours of collection for optimal isolation of the requested cell line(s). Increase the amount of blood/bone marrow submitted based on the number of cell lines requested and for patients with low cell counts.
Ambient.
Clotted or hemolyzed specimens.
Post-transplantation genotypes will be compared to pre-transplant recipient and donor genotypes. Therefore, donor and recipient specimens must be obtained and genotyped before the transplant event occurs. Please provide the results and date of the patient's most recent WBC and differential counts. When submitting bone marrow specimens for cell sorting, please provide information regarding the general cellularity of the patient's bone marrow. See Cell Isolation Request for Chimerism, Post-Transplant, Sorted Cells.
Ambient: 48 hours; Refrigerated: 48 hours; Frozen: Unacceptable
Type Donor - Donor cells only.
Type Recipient - Recipient cells only.
Mixed - Donor and Recipient cells present. Semi-quantitative results of percentage of donor and recipient cells will be reported.
Background Information for Chimerism, Post-Transplant, Sorted Cells:
Indication: Monitoring for bone marrow transplant patients; correlation with clinical status and consideration of the interval between bone marrow transplantation and testing is necessary for proper interpretation of results.
Methodology: PCR followed by capillary electrophoresis. Specimens are analyzed using 15 autosomal markers (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, THO1, D13S317, D16S539, D2S1338, D19S433, vWa, TPOX, D18S51, D5S818, and FGA) and one gender marker (amelogenin).
Kit Used: AmpFLSTR Identifiler® PCR Amplification Kit, Applied Biosystems.
Limit of Detection: 2 percent of minor cell population.
Laboratory Developed Test (LDT)
If requesting multiple cell lines, additional charges apply and vary based on the cell line requested (BMT Cell Isolation or BMT Chimerism Sort). Chimerism, Post-Transplant, Sorted Cells will be billed as separate tests for each cell line requested.
81268; If cell sorting is performed, add 88184 or 88184; 88185
| Component Test Code* | Component Chart Name | LOINC |
|---|---|---|
| 0051670 | Chimerism,Post-Cell Sort, Interpretation | |
| 2001380 | Chimerism,Post-Cell Sort, Specimen | |
| 2002034 | Chimerism,Post-Cell Sort,Margin of Error | |
| 2002035 | Chimerism,Post-Cell Sort, Percent Recip | |
| 2002036 | Chimerism,Post-Cell Sort, Percent Donor | |
| 2002037 | Chimerism,Post-Cell Sort,InformativeLoci |
- Bone Marrow DNA typing
- Bone Marrow DNA Typing Transplant Eval., Post-Transplant
- Bone Marrow Post-Trans Eval
- Bone Marrow Transplantation; Chimerism Evaluation
- Engraftment Monitoring
- Transplant Analysis
- Transplant Evaluation
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