Chimerism, Posttransplant, Sorted Cells (CD 56+ Cells)
Use to monitor engraftment of donor cells post allogeneic stem cell transplant. Each posttransplant specimen and test ordered must have a unique ARUP accession number.
Polymerase Chain Reaction (PCR)/Fragment Analysis/Fluorescence-Activated Cell Sorting (FACS)
New York DOH Approval Status
Lavender (EDTA), pink (K2EDTA), or yellow (ACD solution A or B). OR bone marrow in lavender (EDTA).
Transport 2 mL whole blood. (Min: 2 mL) OR 1 mL bone marrow (Min: 1 mL). Ship overnight. If cell sorting is required, specimens should be received within 24 hours of collection for optimal isolation of the requested cell line(s).
Refrigerated. Also acceptable: Ambient.
Clotted or hemolyzed specimens.
Posttransplant genotypes will be compared to pretransplant recipient and donor genotypes. Therefore, donor and recipient specimens must be obtained and genotyped before the transplant event occurs. Please provide the results and date of the patient's most recent WBC and differential counts. When submitting bone marrow specimens for cell sorting, please provide information regarding the general cellularity of the patient's bone marrow. See Cell Isolation Request for Chimerism, Posttransplant, Sorted Cells.
Room temperature: 72 hours; Refrigerated: 72 hours; Frozen: Unacceptable
Background Information: Chimerism, Posttransplant, Sorted Cells (CD56+ Cells)
Indication: Monitoring for bone marrow transplant patients; correlation with clinical status and consideration of the interval between bone marrow transplantation and testing is necessary for proper interpretation of results.
Methodology: PCR followed by capillary electrophoresis. Specimens are analyzed using 15 autosomal markers (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, THO1, D13S317, D16S539, D2S1338, D19S433, vWa, TPOX, D18S51, D5S818, and FGA) and one gender marker (amelogenin).
Limit of Detection: 2 percent of minor cell population.
Limitations: Diagnostic errors can occur due to rare sequence variations.
Laboratory Developed Test (LDT)
Type Donor: Donor cells only.
Type Recipient: Recipient cells only.
Mixed: Donor and recipient cells present. Semiquantitative results of percentage of donor and recipient cells will be reported.
|Component Test Code*||Component Chart Name||LOINC|
|3005442||Chimerism Post-CD56, Specimen|
|3005443||Chimerism Post-CD56, InformativeLoc|
|3005444||Chimerism Post-CD56, Percent Recip|
|3005445||Chimerism Post-CD56, Percent Donor|
|3005446||Chimerism Post-CD56, Margin Error|
|3005447||Chimerism Post-CD56, Interpretation|
- Bone Marrow DNA typing
- Bone Marrow DNA Typing Transplant Eval., Post-Transplant
- Bone Marrow Post-Trans Eval
- Bone Marrow Transplantation; Chimerism Evaluation
- Engraftment Monitoring
- Transplant Analysis
- Transplant Evaluation