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Paraneoplastic Pemphigus (Paraneoplastic Autoimmune Multiorgan Syndrome) Screening Antibodies by IIF
Ordering Recommendation
Not a preferred initial test for paraneoplastic pemphigus; refer to Paraneoplastic Pemphigus (Paraneoplastic Autoimmune Multiorgan Syndrome) Expanded Antibody Panel by IIF With ELISA (3016534) for preferred initial combination testing with ELISA for IgG envoplakin antibodies and indirect immunofluorescence for rat bladder and other rodent substrate antibodies. This screening test includes only indirect immunofluorescence with rodent substrates; it may be used if IgG envoplakin antibody ELISA testing has been performed separately and for monitoring antibodies in patients with paraneoplastic pemphigus/paraneoplastic autoimmune multiorgan syndrome who do not have increased IgG envoplakin antibodies. Results from this test should be correlated with Direct Immunofluorescence, Tissue Biopsy (Cutaneous, Mucosal, Epithelial) (0092572), histopathological examination of formalin-fixed tissue, testing for IgG envoplakin antibodies if performed separately [refer to Envoplakin Antibody, IgG by ELISA (3016533)], and assessment of other epithelial antibodies [refer to lmmunobullous Disease Antibody Panel (3001409) for comprehensive supplemental testing].
For more information about components, methodology, and specimen type, refer to the ARUP Immunobullous Disease Testing Comparison tool.
New York DOH Approval Status
Specimen Required
Plain red or serum separator tube (SST).
Transfer 2 mL serum to an ARUP standard transport tube. (Min: 0.5 mL)
Refrigerated/ambient
Hemolyzed or lipemic specimens. Plasma.
As a general rule, serum specimens should be shipped to the laboratory as soon as possible. Store refrigerated unless shipping promptly (within 2 hours). Transport at ambient temperature to arrive within 7 days. If 7-14 days until received in laboratory, store and ship refrigerated. If greater than 14 days, serum specimens must be stored and shipped frozen.
Ambient: 1 week; Refrigerated: 2 weeks; Frozen: Indefinitely
Methodology
Semi-Quantitative Indirect Immunofluorescence (IIF)
Performed
Varies
Reported
3-9 days
Reference Interval
By report
Interpretive Data
Refer to report
Performed by non-ARUP Laboratory
Note
The methodology is indirect immunofluorescence (IIF) of patient serum with rodent substrates including rat bladder, mouse bladder, mouse heart, and mouse liver to detect characteristic antibody reactivity: simple columnar epithelial cell surface and basement membrane zone in bladders, intercalated discs in heart, and portal tracts in liver. This test does NOT include IgG envoplakin antibody ELISA testing. Monkey esophagus substrate is included if other concurrent IIF testing does not. For specimens less than 0.5 mL, call the Immunodermatology Laboratory at 801-581-7139.
This test should be distinguished from antibody testing of cerebral spinal fluid (CSF) for paraneoplastic neurologic syndromes; 3004510, 3004512, 3004517 are different tests.
Hotline History
Hotline History
CPT Codes
88346; 88350 x4
Components
| Component Test Code* | Component Chart Name | LOINC |
|---|---|---|
| 0092107 | Paraneoplastic Pemphigus Ab Screen | 42936-5 |
| 3004041 | EER Paraneoplastic Pemphigus Ab Screen | 10865-4 |
Aliases
- cell surface and basement membrane zone antibodies
- envoplakin antibodies
- intercellular substance
- mixed epithelial antibodies
- paraneoplastic autoimmune multiorgan syndrome (PAMS)
- periplakin antibodies
- PNP
- rat bladder immunofluorescence
- Paraneoplastic Pemphigus Antibody Screen
- rat bladder test
