Ordering Recommendation

Monitor previously established MPO/PR3 antibodies or confirm an IFA ANCA  positive test result. Test does not include ANCA testing by IFA.
For  ANCA  testing, refer to the available panel tests. For the workup of suspected vasculitis, refer to ANCA-Associated Vasculitis Profile (ANCA/MPO/PR3) with Reflex to ANCA Titer (2006480). For patients with a history of vasculitis, refer to Anti-Neutrophil Cytoplasmic Antibody with Reflex to Titer and MPO/PR3 Antibodies (2002068).

Mnemonic
MPO/PR3
Methodology

Semi-Quantitative Multiplex Bead Assay

Performed

Sun-Sat

Reported

1-2 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.2 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Plasma, urine, or other body fluids.  Contaminated, hemolyzed, or severely lipemic specimens.

Remarks
Stability

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Reference Interval
Test Number
Components
Reference Interval
0050526 Myeloperoxidase (MPO) Antibody
Negative 19 AU/mL or less
Equivocal 20-25 AU/mL
Positive 26 AU/mL or greater

0050527 Serine Proteinase 3 (PR3) Antibody
Negative 19 AU/mL or less
Equivocal 20-25 AU/mL
Positive 26 AU/mL or greater

Interpretive Data

Approximately 90% of patients with a P-ANCA pattern by IFA have antibodies specific for MPO. 
Approximately 85% of patients with a C-ANCA pattern by IFA have antibodies specific for PR3.

Compliance Category

FDA

Note
Hotline History
N/A
CPT Codes

83516 x2

Components
Component Test Code* Component Chart Name LOINC
0050526 Myeloperox Antibodies, IgG 6969-0
0050527 Serine Proteinase 3, IgG 6968-2
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • 3-ANCA
  • ANCA Antibodies
  • Anticytoplasmic Autoantibodies
  • c-ANCA
  • MPO
  • MPO-ANCA
  • Myeloperoxidase Antibodies
  • p-ANCA
  • PR3
  • PR3-ANCA
  • Proteinase 3 Antibodies
  • Serine Proteinase 3
MPO/PR3 (ANCA) Antibodies