For suspected and/or active herpes simplex virus (HSV) infection, molecular testing is preferred (refer to Herpes Simplex Virus by PCR (0060041)). If pursuing antibody testing, refer to Herpes Simplex Virus Type 1 Glycoprotein G-Specific Antibody, IgG by ELISA, CSF (0050379) and Herpes Simplex Virus Type 2 Glycoprotein G-Specific Antibody, IgG by ELISA, CSF (0050359). If acute HSV infection is suspected, molecular testing is preferred (refer to Herpes Simplex Virus by PCR (0060041)).
Semi-Quantitative Chemiluminescent Immunoassay
Within 24 hours
Transfer 1 mL CSF to an ARUP Standard Transport Tube. (Min: 0.5 mL)
Specimens types other than CSF. Contaminated, heat-inactivated or hemolyzed specimens.
Indicate source on test request form.
Ambient: 8 hours; Refrigerated: 2 weeks; Frozen: 1 year
|0.89 IV or less||Negative - No significant level of detectable HSV IgG antibody.|
|0.90-1.09 IV||Equivocal - Questionable presence of IgG antibodies. Repeat testing in 10-14 days may be helpful.|
|1.10 IV or greater||Positive - IgG antibody to HSV detected which may indicate a current or past HSV infection.|
The detection of antibodies to herpes simplex virus in CSF may indicate central nervous system infection. However, consideration must be given to possible contamination by blood or transfer of serum antibodies across the blood-brain barrier.
Fourfold or greater rise in CSF antibodies to herpes on specimens at least 4 weeks apart are found in 74-94% of patients with herpes encephalitis. Specificity of the test based on a single CSF testing is not established. Presently PCR is the primary means of establishing a diagnosis of herpes encephalitis.
Laboratory Developed Test (LDT)
|Component Test Code*||Component Chart Name||LOINC|
|0050394||HSV 1/2 Antibody Screen IgG, CSF||13249-8|
- Herpes Simplex Antibodies, IgG
- Herpes Simplex Serology, Serum HSV 1,2
- HSV 1, 2 IgG
- HSV 1,2
- HSV CSF IgG