Ordering Recommendation

For suspected and/or active herpes simplex virus (HSV) infection, molecular testing is preferred (refer to Herpes Simplex Virus by PCR (0060041)). If pursuing antibody testing, refer to Herpes Simplex Virus Type 1 Glycoprotein G-Specific Antibody, IgG by ELISA, CSF (0050379) and Herpes Simplex Virus Type 2 Glycoprotein G-Specific Antibody, IgG by ELISA, CSF (0050359). If acute HSV infection is suspected, molecular testing is preferred (refer to Herpes Simplex Virus by PCR (0060041)).

Mnemonic
HER1/2CSF
Methodology

Semi-Quantitative Chemiluminescent Immunoassay

Performed

Sun-Sat

Reported

Within 24 hours

New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible.
Specimen Required
Patient Preparation
Collect

CSF.

Specimen Preparation

Transfer 1 mL CSF to an ARUP Standard Transport Tube. (Min: 0.5 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Specimens types other than CSF. Contaminated, heat-inactivated or hemolyzed specimens.

Remarks

Indicate source on test request form.

Stability

Ambient: 8 hours; Refrigerated: 2 weeks; Frozen: 1 year

Reference Interval
0.89 IV or less Negative - No significant level of detectable HSV IgG antibody.
0.90-1.09 IV Equivocal - Questionable presence of IgG antibodies. Repeat testing in 10-14 days may be helpful.
1.10 IV or greater Positive - IgG antibody to HSV detected which may indicate a current or past HSV infection.

Interpretive Data

The detection of antibodies to herpes simplex virus in CSF may indicate central nervous system infection. However, consideration must be given to possible contamination by blood or transfer of serum antibodies across the blood-brain barrier.

Fourfold or greater rise in CSF antibodies to herpes on specimens at least 4 weeks apart are found in 74-94% of patients with herpes encephalitis.  Specificity of the test based on a single CSF testing is not established.  Presently PCR is the primary means of establishing a diagnosis of herpes encephalitis.

Compliance Category

Laboratory Developed Test (LDT)

Note
Hotline History
N/A
CPT Codes

86694

Components
Component Test Code* Component Chart Name LOINC
0050394 HSV 1/2 Antibody Screen IgG, CSF 13249-8
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Herpes Simplex Antibodies, IgG
  • Herpes Simplex Serology, Serum HSV 1,2
  • HSV 1, 2 IgG
  • HSV 1,2
  • HSV CSF IgG
Herpes Simplex Virus Type 1 and/or 2 Antibodies, IgG, CSF