Ordering Recommendation

Not a stand-alone test. Molecular testing is preferred (refer to Herpes Simplex Virus by PCR (0060041)).

Mnemonic
HERPIICSF
Methodology

Semi-Quantitative Enzyme-Linked Immunosorbent Assay

Performed

Mon, Wed, Fri

Reported

1-5 days

New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible.
Specimen Required
Patient Preparation
Collect

CSF.

Specimen Preparation

Transfer 0.5 mL CSF to an ARUP Standard Transport Tube. (Min: 0.2 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Specimen types other than CSF. Contaminated, heat-inactivated, or hemolyzed specimens.

Remarks

Indicate source on test request form.

Stability

Ambient: 8 hours; Refrigerated: 2 weeks; Frozen: 1 year

Reference Interval
0.89 IV or less Negative - No significant level of detectable IgG antibody to HSV type 2 glycoprotein G.
0.90-1.10 IV Equivocal - Questionable presence of IgG antibody to HSV type 2.  Repeat testing in 10-14 days may be helpful.
1.11 IV or greater Positive - IgG antibody to HSV type 2 glycoprotein G detected, which may indicate a current or past HSV infection.

Interpretive Data

Individuals infected with HSV may not exhibit detectable IgG antibody to type-specific HSV antigens 1 and 2 in the early stages of infection. Detection of antibody presence in these cases may only be possible using a nontype-specific screening test.

The detection of antibodies to herpes simplex virus in CSF may indicate central nervous system infection. However, consideration must be given to possible contamination by blood or transfer of serum antibodies across the blood-brain barrier.

Fourfold or greater rise in CSF antibodies to herpes on specimens tested at least 4 weeks apart are found in 74-94 percent of patients with herpes encephalitis.  Specificity of the test based on a single CSF testing is not established.  Presently PCR is the primary means of establishing a diagnosis of herpes encephalitis.

Compliance Category

Modified FDA

Note
Hotline History
N/A
CPT Codes

86696

Components
Component Test Code* Component Chart Name LOINC
0050359 HSV Type 2 Antibody IgG, CSF 48401-4
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Herpes Simplex glycoprotein G CSF
  • HerpeSelect
  • HSV 2 typing CSF
  • HSV CSF typing
  • HSV Type 2 IgG Specific Ab
  • HSV-2
Herpes Simplex Virus Type 2 Glycoprotein G-Specific Antibody, IgG by ELISA, CSF