•Assess patient with possible paraneoplastic pemphigus (PNP), a RARE paraneoplastic disease associated with lymphoproliferative disorders/malignancies and severe pemphigus clinical features with characteristic IgG antibodies to rodent substrates.
•If other, more common, types of pemphigus are of diagnostic consideration, order the antibody panel test for pemphigus (0090650
) first or concurrently with this test.
•If IgA paraneoplastic antibody testing is required, request through ARUP Client Services 800-242-2787 option 2.
Indirect Fluorescent Antibody
This test is New York DOH approved.
- Patient Preparation
- Plain red or serum separator tube (SST).
- Specimen Preparation
- Transfer 2 mL serum to an ARUP Standard Transport Tube. (Min: 0.5 mL)
- Storage/Transport Temperature
- Unacceptable Conditions
- Hemolyzed or lipemic specimens.
- Ambient: 1 week; Refrigerated: 2 weeks; Frozen: Indefinitely
The methodology is indirect immunofluorescence (IFA) of patient serum on substrates from rodents including mouse heart, liver, bladder and rat bladder to detect characteristic staining: intercalated discs in heart; portal tracts in liver; and simple columnar epithelium cell surface and basement membrane zone in bladders. For specimens less than 0.5 mL, call the Immunodermatology Laboratory at (866) 266-5699.
|Component Test Code*||Component Chart Name||LOINC|
|0092107||Paraneoplastic Pemphigus Ab Screen||42936-5|
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Performed at UUHC-Immunodermatology