Acceptable initial test for strong suspicion of antiphospholipid syndrome (APS). Order with lupus anticoagulant (LA) (0030181
) and anti-beta-2-glycoprotein 1 (anti-β2
GP1) IgG and IgM (0050321
) assays. Combined IgG and IgM cardiolipin (aCL) antibody test is preferred )0099344
Semi-Quantitative Enzyme-Linked Immunosorbent Assay
This test is New York DOH approved.
- Patient Preparation
- Serum separator tube.
- Specimen Preparation
- Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.3 mL)
- Storage/Transport Temperature
- Unacceptable Conditions
- Plasma or other body fluids. Contaminated, heat-inactivated, hemolyzed, or lipemic specimens.
- After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year
0-12 MPL: Negative
13-19 MPL: Indeterminate
20-80 MPL: Low to Medium Positive
81 MPL or above: High positive
Most patients with antiphospholipid antibody syndrome (APS) have medium or high levels of anticardiolipin IgG and/or IgM antibodies. All positive results should be retested on two or more occasions and at least 12 weeks apart to confirm persistence (J Thromb Haemost. 2006;4:295-306).
MPL = IgM phospholipid units
|Component Test Code*||Component Chart Name|
|0050902||Cardiolipin Antibody IgM|
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, contact interface support at firstname.lastname@example.org.
- aCL Antibody
- aCL IgM
- Anticardiolipin Antibodies