Ordering Recommendation

Acceptable initial test when antiphospholipid syndrome (APS) is strongly suspected. Order with Lupus Anticoagulant Reflexive Panel (0030181) and Beta-2 Glycoprotein 1 Antibodies, IgG and IgM (0050321).

Mnemonic
ANTI-CARD
Methodology

Semi-Quantitative Enzyme-Linked Immunosorbent Assay

Performed

Sun-Sat

Reported

Within 24 hours

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.3 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Plasma or other body fluids. Contaminated, heat-inactivated, hemolyzed, or lipemic specimens.

Remarks
Stability

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Reference Interval
Test Number
Components
Reference Interval
0050901 Cardiolipin Antibody, IgG Effective August 18, 2014
0-14 GPL Negative
15-19 GPL Indeterminate
20-80 GPL Low to Moderately Positive
81 GPL or above High Positive

0050902 Cardiolipin Antibody, IgM Effective August 18, 2014
0-12 MPL Negative
13-19 MPL Indeterminate
20-80 MPL Low to Moderately Positive
81 MPL or above High Positive

Interpretive Data

The persistent presence of IgG and/or IgM cardiolipin (CL) antibodies in moderate or high levels (greater than 40 GPL and/or greater than 40 MPL units or greater than 99th percentile) is a laboratory criterion for the diagnosis of antiphospholipid syndrome (APS). Persistence is defined as moderate or high levels of IgG and/or IgM CL antibodies detected in two or more specimens drawn at least 12 weeks apart (J Throm Haemost. 2006;4:295-306). Lower positive levels of IgG and/or IgM CL antibodies (above cutoff but less than 40 GPL and/or less than 40 MPL units) may occur in patients with the clinical symptoms of APS; therefore, the actual significance of these levels is undefined. Results should not be used alone for diagnosis and must be interpreted in light of APS-specific clinical manifestations and/or other criteria phospholipid antibody tests.

No compliance statements are in use for this test.

Note

GPL = IgG phospholipid units; MPL = IgM phospholipid units

Hotline History
N/A
CPT Codes

86147 x2

Components
Component Test Code* Component Chart Name LOINC
0050901 Cardiolipin Antibody IgG 3181-5
0050902 Cardiolipin Antibody IgM 3182-3
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • ACA
  • aCL
  • aCL Ab
  • aCL antibodies
  • aCL IgG, IgM
  • Anti-Cardiolipin
  • Anti-Phospholipid Antibodies
  • Anticardiolipin
  • Anticardiolipin Antibodies
  • Cardiolip
  • Cardiolipin Antibodies, IgG and IgM, Serum
Cardiolipin Antibodies, IgG and IgM