MPO/PR-3 (ANCA) Antibodies
Ordering Recommendation
Use to monitor previously established MPO/PR-3 antibodies or confirm an IFA c-ANCA or p-ANCA positive test result. Test does not include pANCA or cANCA testing. Refer to ANCA Reflex to Titer and MPO/PR-3 Antibodies (2002068) or ANCA-Associated Vasculites Profile (ANCA/MPO/PR-3) Reflex to ANCA Titer (2006480) for pANCA or cANCA testing.
Submit With Order
Semi-Quantitative Multiplex Bead Assay
1-2 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Serum separator tube.  
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.2 mL)  
Storage/Transport Temperature
Unacceptable Conditions
Plasma, urine, or other body fluids. Contaminated, hemolyzed, or severely lipemic specimens.  
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)  
Reference Interval
Test Number Components Reference Interval
0050526Myeloperoxidase Antibody Negative: 19 AU/mL or less
Equivocal: 20-​25 AU/mL
Positive: 26 AU/mL or greater
0050527Serine Protease 3 Antibody Negative: 19 AU/mL or less
Equivocal: 20-​25 AU/mL
Positive: 26 AU/mL or greater
Interpretive Data
Approximately 90% of patients with a P-ANCA pattern by IFA have antibodies specific for MPO.
Approximately 85% of patients with a C-ANCA pattern by IFA have antibodies specific for PR3.
CPT Code(s)
83516 x2
Component Test Code*Component Chart Name
0050526Myeloperox Antibodies, IgG
0050527Serine Protease3, IgG
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Cross References
  • 3-ANCA
  • ANCA Antibodies
  • Anticytoplasmic Autoantibodies
  • c-ANCA
  • MPO
  • Myeloperoxidase Antibodies
  • p-ANCA
  • PR3
  • PR3-ANCA
  • Proteinase 3 Antibodies
  • Serine Protease 3