Multiple Myeloma Panel by FISH

Fluorescence in situ hybridization (FISH) panel is performed on CD138+ enriched cells (assuming specimen is sufficient for enrichment) for multiple myeloma prognosis-specific genomic abnormalities: 1p (CDKN2C loss/deletion)/1q (CKS1B gain/amplification), 17p (TP53 loss/deletion)/ 17q (NF1) control, t(4;14) (IGH/FGFR3 or NSD2 (MMSET) fusion), t(11;14) (IGH/CCND1 fusion and/or +11), t(14;16) (IGH/MAF fusion), t(14;20) (IGH/MAFB fusion).

When this test is ordered in conjunction with a chromosome analysis, and/or genomic microarray, specimen prioritization for low cellularity samples will be given to FISH>microarray>karyotype due to the need for CD138+ cell enrichment prior to FISH and microarray testing. This could impact successful completion of lower priority tests.

If enrichment fails to yield sufficient CD138+ cells, testing will be performed using unenriched cells, if available.

A processing fee will be charged if this procedure is canceled at the client's request, after the test has been set up, or if the specimen integrity is inadequate to allow a complete analysis.

This test must be ordered using Oncology test request form #43099 or through your ARUP interface.

Contact ARUP Genetics Processing for other specimen types or information and specific collection and transportation instructions.
If chromosome analysis is not performed at ARUP on the same sample, Bone Marrow/PBL Culture Processing Fee (0093271) will be added to account for sample processing, and additional charge will apply. If multiple FISH tests are ordered, 0093271 will only be applied to one of the FISH tests.