Ordering Recommendation

Use to order individual or multiple FISH probes for patients with multiple myeloma.

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation

Non-diluted bone marrow aspirate collected in a heparinized syringe. Also acceptable: Whole blood collected in Green (Sodium Heparin).

Specimen Preparation

Transfer 3 mL bone marrow to a green (sodium heparin) (Min: 1 mL). OR transport 5 mL whole blood (Min: 2 mL)

Storage/Transport Temperature

Room temperature.

Unacceptable Conditions

Paraffin-embedded specimens. Clotted specimens.


Desired FISH probe and pertinent clinical diagnosis required with test order. Testing will not be performed until probe and diagnosis are provided; absence of this information will delay turnaround time.


Ambient: 48 hours; Refrigerated: 48 hours; Frozen: Unacceptable


Fluorescence in situ Hybridization (FISH)




3-10 days

Reference Interval

By report

Interpretive Data

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Analyte Specific Reagent (ASR)


Fluorescence in situ hybridization (FISH) is performed on CD138+ sorted cells (assuming specimen is sufficient for sorting) for multiple myeloma prognosis-specific genomic abnormalities. The comprehensive multiple myeloma panel is available under mnemonic FISHMMP (3002063). The following probes may be ordered individually or in any combination: 1p (CDKN2C) loss/deletion/1q (CKS1B) gain/amplification, 1q (CKS1B) gain/amplification/17p (TP53) loss/deletion, t(4;14) (IGH/FGFR3 and MMSET fusion), 8q (MYC) break apart, t(8;14) (IGH/MYC fusion), +9/9p (JAK2) trisomy/gain, t(11;14) (IGH/CCND1 fusion and/or +11), 13q (D13S319) deletion, 14q (IGH) break apart, t(14;16) (IGH/MAF fusion), t(14;20) (IGH/MAFB fusion), +15/15q,+17/17q (PML/RARA gain).

When this test is ordered in conjunction with a chromosome analysis, specimen prioritization will be given to FISH for the sorting of CD138+ cells. This could impact the successful completion of the chromosome analysis.

If sorting fails to yield sufficient CD138+ cells, testing will be performed using unsorted cells, if available.

If more than one probe is ordered, additional charges will apply.

Contact ARUP Genetics Processing (extension 3301) to add a probe to a current specimen.

Other specimen types may be acceptable, contact Genetics Processing (extension 3301) for specific specimen collection and transportation instructions.

This test must be ordered using Oncology test request form #43099 or through your ARUP interface.

Hotline History


CPT Codes

88271; 88275


Component Test Code* Component Chart Name LOINC
0092615 Chromosome FISH, Interphase 57802-1
3002848 EER Chromosome FISH, Interphase 11526-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • MGUS
  • +15/15q
  • +17/17q (PML/RARA gain)
  • 13q (D13S319) deletion
  • 14q (IGH) break apart
  • 1p/1q
  • 8q (MYC)
  • CCND1/IGH (IGH/CCND1 fusion or +11)
  • CD138
  • CDKN2C (1p loss/deletion)
  • CKS1B (1q gain)
  • JAK2 (+9)
  • MM
  • Multiple myeloma
  • PCN
  • Plasma cell neoplasms
  • t(8;14) (IGH/MYC fusion)
  • TP53 (17p deletion)
FISH, Interphase, CD138+ Cells