Use to order individual or multiple FISH probes for patients with multiple myeloma.
Fluorescence in situ Hybridization
New York DOH Approval Status
Non-diluted bone marrow aspirate collected in a heparinized syringe. Also acceptable: Whole blood collected in Green (Sodium Heparin).
Transfer 3 mL bone marrow to a green (sodium heparin) (Min: 1 mL). OR transport 5 mL whole blood (Min: 2 mL)
Paraffin-embedded specimens. Clotted specimens.
Desired FISH probe and pertinent clinical diagnosis required with test order. Testing will not be performed until probe and diagnosis are provided; absence of this information will delay turnaround time.
Ambient: 48 hours; Refrigerated: 48 hours; Frozen: Unacceptable
This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.
Analyte Specific Reagent (ASR)
Fluorescence in situ hybridization (FISH) is performed on CD138+ sorted cells (assuming specimen is sufficient for sorting) for multiple myeloma prognosis-specific genomic abnormalities. The comprehensive multiple myeloma panel is available under mnemonic FISHMMP (3002063). The following probes may be ordered individually or in any combination: 1q (CKS1B) gain/amplification/17p (TP53) loss/deletion, t(4;14) (IGH/FGFR3 and MMSET fusion), 8q (MYC) break apart, t(8;14) (IGH/MYC fusion), +9/9p (JAK2) trisomy/gain, t(11;14) (IGH/CCND1 fusion and/or +11), 13q (D13S319) deletion, 14q (IGH) break apart, t(14;16) (IGH/MAF fusion), t(14;20) (IGH/MAFB fusion), +15/15q,+17/17q (PML/RARA gain).
When this test is ordered in conjunction with a chromosome analysis, specimen prioritization will be given to FISH for the sorting of CD138+ cells. This could impact the successful completion of the chromosome analysis.
If sorting fails to yield sufficient CD138+ cells, testing will be performed using unsorted cells, if available.
If more than one probe is ordered, additional charges will apply.
Contact ARUP Genetics Processing (extension 3301) to add a probe to a current specimen.
Other specimen types may be acceptable, contact Genetics Processing (extension 3301) for specific specimen collection and transportation instructions.
This test must be ordered using Oncology test request form #43099 or through your ARUP interface.
|Component Test Code*||Component Chart Name||LOINC|
|0092615||Chromosome FISH, Interphase||57802-1|
|3002848||EER Chromosome FISH, Interphase||11526-1|
- +17/17q (PML/RARA gain)
- 13q (D13S319) deletion
- 14q (IGH) break apart
- 8q (MYC)
- CCND1/IGH (IGH/CCND1 fusion or +11)
- CKS1B (1q gain)
- JAK2 (+9)
- Multiple Myeloma
- Plasma cell neoplasms
- t(8;14) (IGH/MYC fusion)
- TP53 (17p deletion)