Use to monitor chimerism and engraftment in recipients of allogeneic hematopoietic stem cell transplant. Aids in evaluating the relative amounts of recipient and donor cells present post transplant. Each posttransplant specimen and test ordered must have a unique ARUP accession number.
Polymerase Chain Reaction/Fragment Analysis
New York DOH Approval Status
Lavender (EDTA), pink (K2EDTA), or yellow (ACD solution A or B). OR bone marrow in lavender (EDTA).
Transport 2 mL whole blood (Min: 1 mL) OR 1 mL bone marrow (Min: 1 mL).
Refrigerated. Also acceptable: Ambient.
Posttransplant results will be compared to pretransplant recipient and donor genotypes, therefore, donor and recipient specimens must be obtained and genotyped before the transplant event occurs.
If cell sorting is required, refer to:
Chimerism, Posttransplant, Sorted Cells (T Cells) (3005393) or
Chimerism, Posttransplant, Sorted Cells (B Cells) (3005401) or
Chimerism, Posttransplant, Sorted Cells (CD33+ Cells) (3005409) or
Chimerism, Posttransplant, Sorted Cells (Granulocytes) (3005417) or
Chimerism, Posttransplant, Sorted Cells (Monocytes) (3005425) or
Chimerism, Posttransplant, Sorted Cells (CD34+ Cells) (3005433) or
Chimerism, Posttransplant, Sorted Cells (56+ Cells) (3005441)
Room temperature: 1 week; Refrigerated: 1 month; Frozen: Unacceptable
Background Information: Chimerism, Posttransplant
Indication: Monitoring for bone marrow transplant patients; correlation with clinical status and consideration of the interval between bone marrow transplantation and testing is necessary for proper interpretation of results.
Methodology: PCR followed by capillary electrophoresis. Specimens are analyzed using 15 autosomal markers (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, THO1, D13S317, D16S539, D2S1338, D19S433, vWa, TPOX, D18S51, D5S818, and FGA) and one gender marker (amelogenin).
Limit of Detection: 2 percent of minor cell population.
Limitations: Diagnostic errors can occur due to rare sequence variations.
This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.
Laboratory Developed Test (LDT)
Type Donor: Donor cells only.
Type Recipient: Recipient cells only.
Mixed: Donor and recipient cells present. Semiquantitative results of percentage of donor and recipient cells will be reported.
|Component Test Code*||Component Chart Name||LOINC|
|3005455||Chimerism Post, Specimen|
|3005456||Chimerism Post, InformativeLoc|
|3005457||Chimerism Post, Percent Recip|
|3005458||Chimerism Post, Percent Donor|
|3005459||Chimerism Post, Margin Error|
|3005460||Chimerism Post, Interpretation|
- Bone marrow DNA typing
- Bone Marrow DNA Typing Transplant Eval., Post-Transplant
- Bone Marrow Post-Trans Eval
- Bone Marrow Transplantation
- Chimerism Evaluation
- Transplant Analysis
- Transplant Evaluation