Ordering Recommendation

Aids in the diagnosis of paraneoplastic neurologic syndromes. 

This test only evaluates for the presence of high-risk paraneoplastic antibodies, independent of neurologic phenotype. Targeted phenotype-specific panels are preferred for the evaluation of autoimmune neurologic disease. Refer to the ARUP Autoimmune Neurology Panel Components tables for phenotype-specific test options.

Testing in both serum and CSF is recommended to maximize diagnostic yield; refer to Paraneoplastic Reflexive Panel (3002929) for serum testing.

New York DOH Approval Status

This test is not New York state approved.

Specimen Required

Patient Preparation


Specimen Preparation

Transfer 2 mL CSF to an ARUP Standard Transport Tube. (Min: 1 mL).

Storage/Transport Temperature


Unacceptable Conditions

Contaminated, heat-inactivated, hemolyzed, or lipemic specimens


Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 month


Semi-Quantitative Indirect Fluorescent Antibody/Qualitative Immunoblot




1-9 days

Reference Interval

Test Number
Reference Interval
  Paraneoplastic Abs (PCCA/ANNA) IgG, CSF None Detected
  CV2.1 Ab IgG CBA-IFA Screen, CSF Less than 1:1
  SOX1 Antibody, IgG by Immunoblot, CSF Negative
  Amphiphysin Antibody, CSF Negative

Interpretive Data

Refer to report 

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the U.S. Food and Drug Administration. This test was performed in a CLIA-certified laboratory and is intended for clinical purposes.

Compliance Category

Laboratory Developed Test (LDT)


Purkinje Cell (PCCA) antibody and Neuronal Nuclear (ANNA) antibody IgG are screened by IFA. If the IFA screen is indeterminate, then a Neuronal Nuclear Antibodies (Hu, Ri, Yo, Tr/DNER) IgG by Immunoblot will be added. If the IFA screen is positive at 1:1 or greater, then a PCCA/ANNA antibodies titer and Neuronal Nuclear Antibodies (Hu, Ri, Yo, Tr/DNER) IgG by Immunoblot will be added. Additional charges apply.
If CV2.1 Antibody IgG Screen by IFA is positive, then CV2.1 Antibody IgG Titer by IFA will be added. Additional charges apply.

Hotline History


CPT Codes

86255 x2; 84182 x2; if reflexed add 86256 and/or 84182 x4; if reflexed add 86256


Component Test Code* Component Chart Name LOINC
2010842 Paraneoplastic Abs (PCCA/ANNA) IgG, CSF 14247-1
3002259 CV2.1 Ab IgG CBA-IFA Screen, CSF 53707-6
3002904 SOX1 Antibody, IgG by Immunoblot, CSF 94383-7
3004511 Amphiphysin Antibody, CSF 57750-2
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


Paraneoplastic Reflexive Panel, CSF