Ordering Recommendation

Aids in the diagnosis of paraneoplastic neurological syndromes associated with ANNA-1 (Hu), ANNA-2 (Ri), PCCA-1 (Yo), PCCA-Tr/DNER, amphiphysin, CV2.1, and SOX1 antibodies. Serum is the preferred specimen; see Paraneoplastic Reflexive Panel (3002929).

Mnemonic

PNSPAN CSF

Methodology

Semi-Quantitative Indirect Fluorescent Antibody/Qualitative Immunoblot

Performed

Wed

Reported

1-9 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation
Collect

CSF.

Specimen Preparation

Transfer 2 mL CSF to an ARUP Standard Transport Tube. (Min: 1 mL).

Storage/Transport Temperature

Refrigerated

Unacceptable Conditions

Contaminated, heat-inactivated, hemolyzed, or lipemic specimens

Remarks
Stability

Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 month

Reference Interval

Test Number
Components
Reference Interval
3002257 CV2.1 Screen by IFA with Reflex to Titer, CSF Less than 1:1
2010841 Paraneoplastic Antibodies (PCCA/ANNA) by IFA with Reflex to Titer and Immunoblot, CSF
Test Number
Components
Reference Interval
  Paraneoplastic Abs (PCCA/ANNA) IgG, CSF Refer to report
  Neuronal Nuclear Ab Titer, IgG CSF Refer to report
  Purkinje Cell Antibody Titer IgG, CSF Refer to report
3004527 Neuronal Nuclear Antibodies (Hu, Ri, Yo, Tr/DNER) IgG by Immunoblot, CSF Refer to report

3004510 Amphiphysin Antibody IgG, CSF Negative
3002885 SOX1 Antibody, IgG by Immunoblot, Serum Negative

Interpretive Data

Refer to report 

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the U.S. Food and Drug Administration. This test was performed in a CLIA-certified laboratory and is intended for clinical purposes.

Compliance Category

Laboratory Developed Test (LDT)

Note

Purkinje Cell (PCCA) antibody and Neuronal Nuclear (ANNA) antibody IgG are screened by IFA. If the IFA screen is indeterminate, then a Neuronal Nuclear Antibodies (Hu, Ri, Yo, Tr/DNER) IgG by Immunoblot will be added. If the IFA screen is positive at 1:1 or greater, then a PCCA/ANNA antibodies titer and Neuronal Nuclear Antibodies (Hu, Ri, Yo, Tr/DNER) IgG by Immunoblot will be added. Additional charges apply.
If CV2.1 Antibody IgG Screen by IFA is positive, then CV2.1 Antibody IgG Titer by IFA will be added. Additional charges apply.

Hotline History

N/A

CPT Codes

86255 x2; 84182 x2; if reflexed add 86256 and/or 84182 x4; if reflexed add 86256

Components

Component Test Code* Component Chart Name LOINC
2010842 Paraneoplastic Abs (PCCA/ANNA) IgG, CSF 14247-1
3002259 CV2.1 Ab IgG Screen, CSF 53707-6
3002904 SOX1 Antibody, IgG by Immunoblot, CSF 94383-7
3004511 Amphiphysin Antibody, CSF 57750-2
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

Paraneoplastic Reflexive Panel, CSF