Ordering Recommendation
Aids in the diagnosis of paraneoplastic neurological syndromes associated with ANNA-1 (Hu), ANNA-2 (Ri), PCCA-1 (Yo), PCCA-Tr/DNER, amphiphysin, and CV2.1 antibodies.
Mnemonic
Methodology
Semi-Quantitative Cell-Based Indirect Fluorescent Antibody/Qualitative Immunoblot
Performed
Wed
Reported
1-9 days
New York DOH Approval Status
Specimen Required
Serum Separator Tube (SST)
Separate from cells ASAP or within 2 hours of collection. Transfer 3 mL serum aliquot to an ARUP Standard Transport Tube. (Min: 1.0 mL)
Refrigerated
Contaminated, heat-inactivated, hemolyzed, or lipemic specimens
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 month
Reference Interval
Test Number |
Components |
Reference Interval |
---|---|---|
Neuronal Antibody (Amphiphysin) | Negative | |
Purkinje Cell/Neuronal Nuclear IgG Scrn | None Detected | |
CV2.1 Antibody IgG Screen by IFA | Less than 1:10 | |
SOX1 Antibody, IgG by Immunoblot, Serum | Negative |
Interpretive Data
Refer to report
Laboratory Developed Test (LDT)
Note
Purkinje Cell (PCCA) antibody and Neuronal Nuclear (ANNA) antibody IgG are screened by IFA. If the IFA screen is indeterminate, then a Neuronal Nuclear Antibodies (Hu, Ri, Yo, Tr/DNER) IgG by Immunoblot will be added. If the IFA screen is positive at 1:10 or greater, then a PCCA/ANNA antibodies titer and Neuronal Nuclear Antibodies (Hu, Ri, Yo, Tr/DNER) IgG by Immunoblot will be added. Additional charges apply. If CV2.1 Antibody IgG Screen by IFA is positive, then CV2.1 Antibody IgG Titer by IFA will be added. Additional charges apply.
Hotline History
Hotline History
CPT Codes
86255 x2; 84182 x2; if reflexed add 86256 and/or 84182 x4; if reflexed add 86256
Components
Component Test Code* | Component Chart Name | LOINC |
---|---|---|
0051709 | Neuronal Antibody (Amphiphysin) | 94385-2 |
2007962 | Purkinje Cell/Neuronal Nuclear IgG Scrn | 61177-2 |
2013959 | CV2.1 Antibody IgG Screen by IFA | 72504-4 |
3002903 | SOX1 Antibody, IgG by Immunoblot, Serum | 82444-1 |