Ordering Recommendation

Aids in the diagnosis of paraneoplastic neurological syndromes associated with ANNA-1 (Hu), ANNA-2 (Ri), PCCA-1 (Yo), PCCA-Tr/DNER, amphiphysin, and CV2.1 antibodies.

Mnemonic
PNS PAN2
Methodology

Semi-Quantitative Indirect Fluorescent Antibody/Qualitative Immunoblot

Performed

Wed

Reported

1-9 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Serum Separator Tube (SST)

Specimen Preparation

Separate from cells ASAP or within 2 hours of collection. Transfer 3 mL serum aliquot to an ARUP Standard Transport Tube. (Min: 1.0 mL)

Storage/Transport Temperature

Refrigerated

Unacceptable Conditions

Contaminated, heat-inactivated, hemolyzed, or lipemic specimens

Remarks
Stability

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 month

Reference Interval
Test Number
Components
Reference Interval
2013956 CV2.1 Screen by IFA with Reflex to Titer Less than 1:10
2007961 Paraneoplastic Antibodies (PCCA/ANNA) by IFA with Reflex to Titer and Immunoblot Effective August 17, 2020
Test Number
Components
Reference Interval
Purkinje Cell/Neuronal Nuclear IgG Scrn None Detected
Neuronal Nuclear Antibody (ANNA) IFA Titer, IgG Less than 1:10
Purkinje Cell Antibody, Titer Less than 1:10
3002917 Neuronal Nuclear Antibodies (Hu, Ri, Yo, Tr/DNER) IgG by Immunoblot, Serum Refer to report

2008893 Amphiphysin Antibody, IgG Negative
3002885 SOX1 Antibody, IgG by Immunoblot, Serum Negative

Interpretive Data

Refer to report

Compliance Category

Laboratory Developed Test (LDT)

Note

Purkinje Cell (PCCA) antibody and Neuronal Nuclear (ANNA) antibody IgG are screened by IFA. If the IFA screen is indeterminate, then a Neuronal Nuclear Antibodies (Hu, Ri, Yo, Tr/DNER) IgG by Immunoblot will be added. If the IFA screen is positive at 1:10 or greater, then a PCCA/ANNA antibodies titer and Neuronal Nuclear Antibodies (Hu, Ri, Yo, Tr/DNER) IgG by Immunoblot will be added. Additional charges apply. If CV2.1 Antibody IgG Screen by IFA is positive, then CV2.1 Antibody IgG Titer by IFA will be added. Additional charges apply.

Hotline History
N/A
CPT Codes

86255 x2; 84182 x2; if reflexed add 86256 and/or 84182 x4; if reflexed add 86256

Components
Component Test Code* Component Chart Name LOINC
0051709 Neuronal Antibody (Amphiphysin) 94385-2
2007962 Purkinje Cell/Neuronal Nuclear IgG Scrn 61177-2
2013959 CV2.1 Antibody IgG Screen by IFA
3002903 SOX1 Antibody, IgG by Immunoblot, Serum
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
Paraneoplastic Reflexive Panel