Ordering Recommendation

Preferred reflex test to detect Lyme disease in individuals with ≤4 weeks of clinical symptoms or exposure to tick. Positive/equivocal screen confirmed by immunoblot.

Mnemonic
LPEP ACUTE
Methodology

Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Qualitative Immunoblot

Performed

Mon-Fri

Reported

1-4 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Serum separator tube.

Specimen Preparation

Transfer 2 mL serum to an ARUP Standard Transport Tube. (Min: 0.15 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Plasma, CSF. Contaminated, heat-inactivated, hemolyzed, icteric, or lipemic specimens.

Remarks
Stability

After separation from cells: Ambient: 48 hours; Refrigerated:10 days; Frozen: 1 month

Reference Interval
Test Number
Components
Reference Interval
3003253 Borrelia burgdorferi VlsE1/pepC10 Antibodies, Total by ELISA
Reference Interval
0.90 IV or less Negative - VlsE1 and pepC10 antibodies to B. burgdorferi not detected.
0.91-1.09 IV Equivocal - Repeat testing in 10-14 days may be helpful.
1.10 IV or greater Positive - VlsE1 and pepC10 antibodies to B. burgdorferi detected.

0050254 Borrelia burgdorferi Antibodies, IgG and IgM by Immunoblot Effective August 15, 2011

Negative

Interpretive Data

Refer to report.

Compliance Category

FDA

Note

If VlsE1/pepC10 antibodies by ELISA is 0.91 IV or greater, then IgG and IgM Immunoblot will be added. Additional charges apply.

Hotline History
N/A
CPT Codes

86618; if reflexed, add 86617 x2

Components
Component Test Code* Component Chart Name LOINC
3003258 B. burgdorferi VlsE1/pepC10 Abs, ELISA 5060-9
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Lyme Disease
  • Lyme Disease C6 Peptide Ab Reflex Panel
  • Lyme Disease VlsE1/pepC10 Ab, ELISA reflex to WB
  • VlsE Lipoprotein, VlsE1/pepC10 Assay
Borrelia burgdorferi VlsE1/pepC10 Antibodies, Total by ELISA with Reflex to IgG and IgM by Immunoblot