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Hemoglobin F with Reflex to Electrophoresis
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Ordering Recommendation
Use to determine percentage of hemoglobin F. For complete hemoglobin evaluation, order Hemoglobin Evaluation with Reflex to Electrophoresis and/or RBC Solubility (0050610).
Mnemonic
Methodology
High Performance Liquid Chromatography (HPLC)/Electrophoresis
Performed
Sun-Sat
Reported
1-3 days
New York DOH Approval Status
Specimen Required
Lavender (EDTA) or pink (K2EDTA).
Transport 3 mL whole blood. (Min: 0.2 mL)
Refrigerated.
Frozen or room temperature specimens.
Ambient: Unacceptable; Refrigerated: 1 week; Frozen: Unacceptable
Reference Interval
Age-Defined Normal Hemoglobin Reference Intervals
| Age | Hgb F Percent |
|---|---|
| 0-1 month | 45.8-91.7 |
| 2 months | 32-7-85.2 |
| 3 months | 14.5-73.7 |
| 4 months | 4.2-56.9 |
| 5 months | 1.0-38.1 |
| 6-8 months | 0.9-19.4 |
| 9-12 months | 0.6-11.6 |
| 13-23 months | 0.0-8.5 |
| 2 years and older | 0.0-2.1 |
Interpretive Data
Refer to report.
FDA
Note
This assay measures percentage of hemoglobin F only. If abnormal peaks suggestive of a hemoglobin variant are detected, then Capillary Electrophoresis will be added to aid in confirmation and identification of the variant. Additional charges apply. For complete hemoglobin evaluation, order Hemoglobin Evaluation with Reflex to Electrophoresis and/or RBC Solubility (0050610).
Hotline History
CPT Codes
83021; if reflexed, add 83020
Components
| Component Test Code* | Component Chart Name | LOINC |
|---|---|---|
| 2008793 | Hemoglobin, Capillary Electrophoresis | 13514-5 |
| 3002710 | Hemoglobin F | 4576-5 |
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