3017101
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Hemoglobin Evaluation With Reflex to Electrophoresis and/or RBC Solubility

HGBEL RFX
Example Reports
Abnormal CE
Abnormal HPLC
Normal

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Patient History for Hemoglobinopathy/Thalassemia Testing

Ordering Recommendation

Effective test to screen for hemoglobinopathies. May be used for follow-up in individuals with a known hemoglobinopathy.

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation
Collect

Lavender (EDTA) or pink (K2EDTA).

Specimen Preparation

Transport 5 mL whole blood. (Min: 0.5 mL)

Storage/Transport Temperature

Refrigerated

Unacceptable Conditions

Frozen or room temperature specimens.

Remarks
Stability

Ambient: Unacceptable; Refrigerated: 1 week; Frozen: Unacceptable

Methodology

High Performance Liquid Chromatography (HPLC) /Electrophoresis/RBC Solubility

Performed

Sun-Sat

Reported

1-5 days

Reference Interval

Test Number
 Components
 Reference Interval
Hemoglobin A Age                   Reference Intervals (%)
0-1 months                    7.6-54.8
2 months                        14.7-70.1
3 months                        26.6-81.8
4 months                        43.0-89.5
5 months                        60.8-94.0
6-8 months                    78.2-96.6
9-12 months                  86.1-97.2
13-23 months                85.1-97.7
2 years and older           95.0-97.9
Hemoglobin A2 Age                Reference Intervals (%)
0-1 months                    0.0-1.4
2 months                        0.0-2.0
3 months                         0.1-2.6
4 months                         0.8-3.0
5 months                         1.5-3.3
6-8 months                     1.8-3.5
9-23 months                   1.9-3.5
2 years and older          2.0-3.5
Hemoglobin F Age                     Reference Intervals (%)
0-1 months                   45.8-91.7
2 months                       32.7-85.2
3 months                       14.5-73.7
4 months                       4.2-56.9
5 months                       1.0-38.1
6-8 months                    0.9-19.4
9-12 months                  0.6-11.6
13-23 months                 0.0-8.5
2 years and older            0.0-2.1
Hemoglobin S 0.0
Hemoglobin C 0.0
Hemoglobin E 0.0
Hemoglobin Other 0.0

Interpretive Data

Sickle Cell Solubility Reflex:

Not Performed: Solubility testing for Hemoglobin S not indicated.
Positive: Positive for Hemoglobin S by HPLC and confirmed by solubility testing. Additional charges apply.
Conf Previous: Positive for Hemoglobin S by HPLC. Solubility testing performed previously and not repeated with this submission.

Hgb Capillary Electrophoresis Reflex:

Not Performed: Confirmation by Capillary Electrophoresis not indicated.
Performed: Results confirmed by Capillary Electrophoresis. Additional charges apply.
Conf Previous: Capillary Electrophoresis confirmation performed as part of a previous submission. Confirmation not repeated with this submission.

Compliance Category

Modified FDA

Note

If abnormal peaks suggestive of a hemoglobin variant are detected, then RBC Solubility and/or Capillary Electrophoresis will be performed to aid in confirmation and identification of the variant. Additional charges apply.

If a hemoglobin variant cannot be quantitated by HPLC, results from capillary electrophoresis will be reported.

Quantitation of hemoglobin is recommended for a definitive diagnosis in infants 1 year and older.

Hotline History

View Hotline History

 

Hotline History

Date of Change
Test Name Change
Methodology
Performance/Reported Schedule
Specimen Requirements
Reference Interval
Interpretive Data
Note
CPT Code
Component Change
Other Interface Change
New Test
Inactive
02/20/2024
X
N/A

CPT Codes

83021; if reflexed, add 83020; 85660

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Components

Component Test Code* Component Chart Name LOINC
3005529 Hemoglobin A 4547-6
3005665 Hemoglobin A2 4551-8
3005666 Hemoglobin F 4576-5
3005667 Hemoglobin S 4625-0
3005668 Hemoglobin C 4563-3
3005669 Hemoglobin E 4575-7
3005670 Hemoglobin Other 48343-8
3005671 Hemoglobin Evaluation 21026-0
3005673 Sickle Cell Solubility Reflex 6864-3
3017105 Hgb Capillary Electrophoresis Reflex 13514-5
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • Evaluation, Hemoglobin
  • Fetal Hemoglobin (Hemoglobin F)
  • Hb
  • Hb ELP
  • Hb IEF
  • Hemoglobin Evaluation
  • Hemoglobin Fractionation
  • Hemoglobinopathy Fractionation Profile
  • Hgb
  • Sickle Cell Anemia Screen
  • Sickle Cell Disease
  • Sickle Cell Screen
Hemoglobin Evaluation With Reflex to Electrophoresis and/or RBC Solubility