Ordering Recommendation

Aids in the diagnosis and monitoring of multiple myeloma, some B cell lymphoproliferative disorders, Waldenstrom's macroglobulilnemia, AL amyloidosis, and light chain deposition disease in conjunction with other laboratory and clinical findings. Identifies, quantitates, and characterizes monoclonal protein. Useful in initial diagnosis and throughout the treatment phase of patients with monoclonal gammopathy disorders. Preferred test is Protein Electrophoresis with Reflex to Immunofixation, Serum (2002109).

Mnemonic
IFE SPEP
Methodology

Qualitative Immunofixation Electrophoresis/Quantitative Capillary Electrophoresis/Quantitative Spectrophotometry

Performed

Sun-Sat

Reported

1-5 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Serum Separator Tube (SST).

Specimen Preparation

Separate from cells ASAP or within 2 hours of collection. Transfer 1.5 mL serum to an ARUP Standard Transport Tube. (Min: 1 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Plasma.

Remarks
Stability

After separation from cells: Ambient: Unacceptable; Refrigerated: 1 week; Frozen: 1 month

Reference Interval
Test Number
Components
Reference Interval
0050640 Protein Electrophoresis, Serum Effective August 19, 2019
Test Number
Components
Reference Interval
  Total Protein, Serum Refer to report
  Albumin Refer to report
  Alpha 1 Globulin Refer to report
  Alpha 2 Globulin Refer to report
  Beta Globulin Refer to report
  Gamma Refer to report

Interpretive Data



Compliance Category

FDA

Note

A copy of the graph will follow the final report.

Hotline History
N/A
CPT Codes

84155; 84165; 86334

Components
Component Test Code* Component Chart Name LOINC
0050020 Albumin 2862-1
0050035 Alpha 1 Globulin 2865-4
0050060 Alpha 2 Globulin 2868-8
0050110 Beta Globulin 2871-2
0050270 Gamma 2874-6
0050271 Immunofixation 25700-6
0050545 Total Protein, Serum 2885-2
2005168 SPEP/IFE Interpretation 49275-1
3002569 EER Monoclonal Protein Study, Serum
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • IFE
  • Immunoelectrophoresis (Monoclonal Protein Detection Quantitation and Characterization, SPEP, IFE, Se
  • Immunofixation, serum
  • MGUS study
  • Monoclonal Protein Study
  • Multiple Myeloma Study
  • Protein ELP
  • Serum Protein Electrophoresis
  • SPEP
Monoclonal Protein Study, Serum