Ordering Recommendation

Aids in the diagnosis and monitoring of multiple myeloma, some B cell lymphoproliferative disorders, Waldenstrom's macroglobulilnemia, AL amyloidosis, and light chain deposition disease in conjunction with other laboratory and clinical findings. Identifies, quantitates, and characterizes monoclonal protein. Useful in initial diagnosis and throughout the treatment phase of patients with monoclonal gammopathy disorders. Preferred test is Protein Electrophoresis with Reflex to Immunofixation, Serum (2002109).

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation

Serum separator tube (SST).

Specimen Preparation

Separate from cells ASAP or within 2 hours of collection. Transfer 1.5 mL serum to an ARUP standard transport tube. (Min: 1 mL)

Storage/Transport Temperature


Unacceptable Conditions



After separation from cells: Ambient: Unacceptable; Refrigerated: 1 week; Frozen: 1 month


Qualitative Immunofixation Electrophoresis/Quantitative Capillary Electrophoresis/Colorimetry




1-5 days

Reference Interval

Test Number
Reference Interval
  Albumin 3.75-5.01 g/dL
  Alpha 1 Globulin 0.19-0.46 g/dL
  Alpha 2 Globulin 0.48-1.05 g/dL
  Beta Globulin 0.48-1.10 g/dL
  Gamma 0.62-1.51 g/dL
  Total Protein, Serum Refer to report. Reference intervals may vary based on instrumentation.
  Monoclonal Protein <=0.00 g/dL

Interpretive Data

Compliance Category



A copy of the graph will follow the final report.

Hotline History


CPT Codes

84155; 84165; 86334


Component Test Code* Component Chart Name LOINC
0050020 Albumin 2862-1
0050035 Alpha 1 Globulin 2865-4
0050060 Alpha 2 Globulin 2868-8
0050110 Beta Globulin 2871-2
0050270 Gamma 2874-6
0050271 Immunofixation 25700-6
0050545 Total Protein, Serum 2885-2
2005168 SPEP/IFE Interpretation 49275-1
2011827 Monoclonal Protein 33358-3
3002569 EER Monoclonal Protein Study, Serum 11526-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • IFE
  • Immunoelectrophoresis (Monoclonal Protein Detection Quantitation and Characterization, SPEP, IFE, Se
  • Immunofixation, serum
  • MGUS study
  • Monoclonal Protein Study
  • Multiple Myeloma Study
  • Protein ELP
  • Serum Protein Electrophoresis
  • SPEP
Monoclonal Protein Study, Serum