Protein Electrophoresis with Reflex to Immunofixation, Serum

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Ordering Recommendation

Aid in the diagnosis and management of monoclonal gammopathies. Use to detect and quantify serum monoclonal protein. Immunofixation is performed if patterns observed in protein electrophoresis p are monoclonal or suspicious.

Mnemonic

SPEP REFLEX

Methodology

Quantitative Capillary Electrophoresis/Qualitative Immunofixation Electrophoresis/Quantitative Immunoturbidimetry/Colorimetry

Performed

Sun-Sat

Reported

1-5 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation
Collect

Serum Separator Tube (SST).

Specimen Preparation

Separate from cells ASAP or within 2 hours of collection. Transfer 1.5 mL serum to an ARUP Standard Transport Tube. (Min: 1.5 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Plasma.

Remarks
Stability

After separation from cells: Ambient: Unacceptable; Refrigerated: 1 week; Frozen: 1 month

Reference Interval

Test Number
 Components
 Reference Interval
  Albumin 3.75-5.01 g/dL
  Alpha 1 Globulin 0.19-0.46 g/dL
  Alpha 2 Globulin 0.48-1.05 g/dL
  Beta Globulin 0.48-1.10 g/dL
  Gamma 0.62-1.51 g/dL
  Total Protein, Serum Refer to report. Reference intervals may vary based on instrumentation.

Interpretive Data

Serum protein electrophoresis, when used as a screening test, is useful in the detection of various pathophysiologic states such as inflammation, protein loss, gammopathies, and other dysproteinemias. However, immunofixation electrophoresis (IFE) is a more sensitive technique for the identification of small M-proteins found in patients with amyloidosis, early or treated myeloma or macroglobulinemia, solitary plasmacytoma, or extramedullary plasmacytoma.

Compliance Category

FDA

Note

If patterns from serum protein electrophoresis are monoclonal or suspicious, then Immunofixation Electrophoresis will be added. Additional charges apply.

No additional testing will be added for proteins that are normal, polyclonal, or that lack monoclonal patterns.

Hotline History

View Hotline History

 

Hotline History

Date of Change
Test Name Change
Methodology
Performance/Reported Schedule
Specimen Requirements
Reference Interval
Interpretive Data
Note
CPT Code
Component Change
Other Interface Change
New Test
Inactive
08/15/2022
X
02/16/2021
X
X
N/A

CPT Codes

84155; 84165; if reflexed, add 82784 x3; 86334

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Components

Component Test Code* Component Chart Name LOINC
0050020 Albumin 2862-1
0050035 Alpha 1 Globulin 2865-4
0050060 Alpha 2 Globulin 2868-8
0050110 Beta Globulin 2871-2
0050270 Gamma 2874-6
0050346 Immunofixation Reflex 13348-8
0050545 Total Protein, Serum 2885-2
2005168 SPEP/IFE Interpretation 49275-1
2011827 Monoclonal Protein 33358-3
2012455 EER Serum Protein Electrophoresis Reflex 11526-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • Electrophoresis, Protein, Serum with Reflex to Immunofixation Electrophoresis
  • IFE Reflex
  • MGUS study
  • Monoclonal protein detection
  • Monoclonal Protein Study
  • Multiple Myeloma Study
  • Protein Electrophoresis with Interpretation, Serum and Reflex to IFE and QNTIG's
  • Protein Electrophoresis, Serum with Reflex to IFE, Serum
  • Protein Electrophoresis, with Interpretation Serum, With Reflex to IFE, Serum
  • SPEP
Protein Electrophoresis with Reflex to Immunofixation, Serum