Ordering Recommendation

Aid in the diagnosis and management of monoclonal gammopathies. Use to detect and quantify serum monoclonal protein. Immunofixation is performed if patterns observed in protein electrophoresis p are monoclonal or suspicious.




Quantitative Capillary Electrophoresis/Qualitative Immunofixation Electrophoresis/Quantitative Immunoturbidimetry/Quantitative Spectrophotometry




1-5 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation

Serum Separator Tube (SST).

Specimen Preparation

Separate from cells ASAP or within 2 hours of collection. Transfer 1.5 mL serum to an ARUP Standard Transport Tube. (Min: 1.5 mL)

Storage/Transport Temperature


Unacceptable Conditions



After separation from cells: Ambient: Unacceptable; Refrigerated: 1 week; Frozen: 1 month

Reference Interval

Test Number
Reference Interval

0050640 Protein Electrophoresis, Serum Effective August 19, 2019
Test Number
Reference Interval
  Total Protein, Serum Refer to report
  Albumin Refer to report
  Alpha 1 Globulin Refer to report
  Alpha 2 Globulin Refer to report
  Beta Globulin Refer to report
  Gamma Refer to report

0050350 Immunoglobulin G Effective February 16, 2021
0-2 years: 242-1108 mg/dL            
3-4 years: 485-1160 mg/dL
5-9 years: 514-1672 mg/dL     
10-14 years: 581-1652 mg/dL                   
15-18 years: 479-1433 mg/dL                         
19 years and older : 768-1632 mg/dL

0050340 Immunoglobulin A Effective February 16, 2021
0-2 years: 2-126 mg/dL
3-4 years: 14-212 mg/dL
5-9 years: 52-226 mg/dL
10-14 years: 42-345 mg/dL
15-18 years: 60-349 mg/dL
19 years and older: 68-408 mg/dL

0050355 Immunoglobulin M Effective February 16, 2021
0-2 years: 21-215 mg/dL                     
3-4 years: 26-155 mg/dL                                
5-9 years: 26-188 mg/dL                          
10-14 years: 47-252 mg/dL                               
15-18 years: 26-232 mg/dL
19 years and older: 35-263 mg/dL

Interpretive Data

Serum protein electrophoresis, when used as a screening test, is useful in the detection of various pathophysiologic states such as inflammation, protein loss, gammopathies, and other dysproteinemias. However, immunofixation electrophoresis (IFE) is a more sensitive technique for the identification of small M-proteins found in patients with amyloidosis, early or treated myeloma or macroglobulinemia, solitary plasmacytoma, or extramedullary plasmacytoma.

Compliance Category



If patterns from serum protein electrophoresis are monoclonal or suspicious, then Immunofixation Electrophoresis will be added. Additional charges apply.

No additional testing will be added for proteins that are normal, polyclonal, or that lack monoclonal patterns.

Hotline History


CPT Codes

84155; 84165; if reflexed, add 82784 x3; 86334


Component Test Code* Component Chart Name LOINC
0050020 Albumin 2862-1
0050035 Alpha 1 Globulin 2865-4
0050060 Alpha 2 Globulin 2868-8
0050110 Beta Globulin 2871-2
0050270 Gamma 2874-6
0050346 Immunofixation Reflex 13348-8
0050545 Total Protein, Serum 2885-2
2005168 SPEP/IFE Interpretation 49275-1
2012455 EER Serum Protein Electrophoresis Reflex 11526-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • Electrophoresis, Protein, Serum with Reflex to Immunofixation Electrophoresis
  • IFE Reflex
  • MGUS study
  • Monoclonal protein detection
  • Monoclonal Protein Study
  • Multiple Myeloma Study
  • Protein Electrophoresis with Interpretation, Serum and Reflex to IFE and QNTIG's
  • Protein Electrophoresis, Serum with Reflex to IFE, Serum
  • Protein Electrophoresis, with Interpretation Serum, With Reflex to IFE, Serum
  • SPEP
Protein Electrophoresis with Reflex to Immunofixation, Serum