Ordering Recommendation

Useful in combination with Factor VIII, Activity (0030095) for the initial diagnosis of mild hemophilia A. May also be used to measure factor VIII activity in the presence of a lupus anticoagulant. Monitor certain extended half-life factor VIII replacement products and measure native factor VIII or factor VIII concentrates in patients on emicizumab (Hemlibra).

Mnemonic
CHROM F8
Methodology

Chromogenic

Performed

Mon, Wed, Fri

Reported

1-4 days

New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible.
Specimen Required
Patient Preparation
Collect

Light Blue (Sodium Citrate). Special Specimen Collection and Handling Hemostasis/Thrombosis Specimens guide located at https://www.aruplab.com/Specimen-Handling/SpecialSpecimenCollection/Hemostasis-Thrombosis.pdf

Specimen Preparation

Transfer 1 mL platelet-poor plasma to an ARUP Standard Transport Tube. (Min: 0.8 mL)

Storage/Transport Temperature

CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.

Unacceptable Conditions

Serum or EDTA plasma. Clotted or hemolyzed specimens.

Remarks
Stability

Ambient: 4 hours; Refrigerated: Unacceptable; Frozen at -20° C: 3 months; Frozen at -70° C: 6 months

Reference Interval
Age Reference Interval
0-6 years 56-191 percent
7-9 years 76-199 percent
10-11 years 80-209 percent
12-13 years 72-198 percent
14-15 years 69-237 percent
16-17 years 63-221 percent
18 years and older 56-191 percent

Interpretive Data

Information on the clinical uses of chromogenic FVIII activity testing can be found at arupconsult.com.

Compliance Category

Laboratory Developed Test (LDT)

Note
Hotline History
N/A
CPT Codes

85240

Components
Component Test Code* Component Chart Name LOINC
3002344 Chromogenic Factor VIII, Activity 49865-9
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • ADYNOVATE, AFSTYLA, ELOCTATE
  • AHF chromogenic
  • Antihemophilic Factor (AHF) chromogenic
  • Coagulation Factor VIII Chromogenic activity assay, plasma
  • Factor VIII activity, chromogenic
  • Factor VIII chromogenic assay
  • Factor VIII:C chromogenic
  • Hemlibra; emicizumab, extended half-life factor VIII products
Chromogenic Factor VIII, Activity