Immunofixation with Free Light Chains, Quantitative, Urine

3002104
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Ordering Recommendation

Aid in the monitoring of patients with urinary monoclonal proteins (Bence Jones protein).

Mnemonic

U IFE FLC

Methodology

Qualitative Immunofixation Electrophoresis/Quantitative Immunoturbidimetry

Performed

Sun-Sat

Reported

1-5 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation
Collect

24-hour urine. Refrigerate during collection. Also acceptable: Random urine specimens and urine supernate.

Specimen Preparation

Transfer two 4 mL aliquots from a well-mixed 24-hour collection to individual ARUP Standard Transport Tubes. (Min: 4 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions
Remarks

Record total volume and collection time interval on transport tube and test request form.

Stability

Ambient: Unacceptable; Refrigerated: 3 weeks; Frozen: 6 months

Reference Interval

Test Number
 Components
 Reference Interval
  Total Protein Less than 150 mg/d
  Free Urinary Kappa Light Chains 0.00 - 32.90 mg/L
  Free Urinary Kappa Excretion/Day By report
  Free Urinary Lambda Light Chain 0.00 - 3.79 mg/L
  Free Urinary Lambda Excretion/Day By report
  IFE Interpretation By report

Interpretive Data

Results of urine free light chain testing can be used to monitor disease progression or response to therapy in patients for whom urine electrophoresis is unable to provide reliable Bence Jones Protein quantification. The results of urine kappa and lambda free light chain quantitative values may be misleading in specimens with high levels of urinary polyclonal free light chains, and absent Bence Jones protein by immunofixation; therefore correlation with urine immunofixation is required to identify inconsistent results.

Total urinary protein is determined turbidimetrically by adding the albumin and kappa and/or lambda light chains. This value may not agree with the total protein as determined by chemical methods, which characteristically underestimates urinary light chains.

Undetected antigen excess is a rare event but cannot be excluded. Free light chain results should always be interpreted in conjunction with other clinical and laboratory findings.

Compliance Category

FDA

Note

Hotline History

N/A

CPT Codes

84156; 86335; 83521 x2

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Components

Component Test Code* Component Chart Name LOINC
0050565 Total Protein 2889-4
0050683 Free Urinary Kappa Light Chains 38176-4
0050684 Free Urinary Kappa Excretion/Day 38177-2
0050685 Free Urinary Lambda Light Chain 38178-0
0050686 Free Urinary Lambda Excretion/Day 38169-9
0050688 IFE Interpretation 49277-7
0097110 Total Volume 19153-6
0097111 Hours Collected 30211-7
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • Free Urinary Kappa Light Chains
  • Free Urinary Kappa/Lambda Ratio
  • Free Urinary Lambda Light Chain
  • IFE
  • IFE urine
  • Immunofixation Electrophoresis, urine
  • Monoclonal protein detection and quantitation, urine
  • Urinary Light Chains
Immunofixation with Free Light Chains, Quantitative, Urine