Ordering Recommendation

Aid in the diagnosis and monitoring of monoclonal gammopathies using 24 hours urine specimens.

Mnemonic
U-PEP
Methodology

Semi-Quantitative Electrophoresis/Qualitative Immunofixation Electrophoresis

Performed

Mon-Fri

Reported

1-5 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

24-hour urine. Refrigerate during collection. Also acceptable: Random urine specimens and urine supernate.

Specimen Preparation

Transfer two 4 mL aliquots from well-mixed 24 hour collection to individual ARUP Standard Transport Tubes. (Min: 4 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions
Remarks

Record total volume and collection time interval on transport tube and test request form.

Stability

Ambient: Unacceptable; Refrigerated: 1 week; Frozen: 1 month

Reference Interval
Test Number
Components
Reference Interval
Total Protein, Urine mg/dL Not established
Urine 24 Hour Protein 40 - 150 mg/d
Albumin%, Urine Not established
Alpha-1 Globulins%, Urine Not established
Alpha-2 Globulins%, Urine Not established
Beta Globulins%, Urine Not established
Gamma Globulins%, Urine Not established
Paraprotein%, Urine Not established
Paraprotein Excretion mg/dL Not established
IFE Interpretation By report

Interpretive Data

Total urine protein measurement using this method characteristically underestimates urinary light chains.

Compliance Category

FDA

Note
Hotline History
N/A
CPT Codes

84156; 84166; 86335

Components
Component Test Code* Component Chart Name LOINC
0020200 Total Protein, Urine-per volume 2888-6
0020215 Urine 24 Hour Protein 2889-4
0050688 IFE Interpretation 49277-7
0097110 Total Volume 19153-6
0097111 Hours Collected 30211-7
2002465 Paraprotein Excretion/24 Hour 42482-0
2002466 Alpha-1 %, Urine 17811-1
2002467 Alpha-2 %, Urine 17813-7
2002468 Beta Globulin %, Urine 17815-2
2002469 Gamma Globulin %, Urine 17817-8
2002478 Albumin %, Urine 17819-4
2002490 Paraprotein %, Urine 42484-6
3002262 EER Monoclonal Protein Study, 24 Hour U 11526-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Electrophoresis and Immunofixation, urine
  • IFE
  • Light Chain Disease Study
  • Monoclonal Protein Study, urine
  • Protein Electrophoresis, Urine
  • Protein Fractionation, urine
  • UEP
  • UPEP
  • Urine IFE
  • Urine Protein Electrophoresis
Monoclonal Protein Study, 24 hour, Urine