Aids in risk stratification of individuals with multiple myeloma. Recommended at initial diagnosis and in standard or low-risk individuals at time of disease progression.
Fluorescence in situ Hybridization
Non-diluted bone marrow collected in a heparinized syringe. Also acceptable: Green (sodium heparin).
Transfer 3 mL bone marrow to a green (sodium heparin) (Min: 1 mL). OR transport 5 mL whole blood (Min: 2 mL).
Frozen specimens. Paraffin-embedded specimens. Clotted specimens.
Ambient: 48 hours; Refrigerated: 48 hours; Frozen: Unacceptable
Analyte Specific Reagent (ASR)
Fluorescence in situ hybridization (FISH) panel is performed on CD138+ sorted cells (assuming specimen is sufficient for sorting) for multiple myeloma prognosis-specific genomic abnormalities: 1q (CKS1B) gain/amplification/17p (TP53) loss/deletion, t(4;14) (IGH/FGFR3 and MMSET fusion)/+9/9q (ASS1) trisomy/gain, t(11;14) (IGH/CCND1 fusion and/or +11), t(14;16) (IGH/MAF fusion), t(14;20) (IGH/MAFB fusion).
When this test is ordered in conjunction with a chromosome analysis, specimen prioritization will be given to FISH for the sorting of CD138+ cells. This could impact the successful completion of the chromosome analysis.
If sorting fails to yield sufficient CD138+ cells, testing will be performed using unsorted cells, if available.
A processing fee will be charged if this procedure is canceled at the client's request, after the test has been set up, or if the specimen integrity is inadequate to allow a complete analysis.
This test must be ordered using Oncology test request form #43099 or through your ARUP interface.
Contact ARUP Genetics Processing for other specimen types or information and specific collection and transportation instructions.
88271 x7; 88275 x7; 88291
|Component Test Code*||Component Chart Name||LOINC|
|0092617||Multiple Myeloma Panel by FISH||57802-1|
|3002592||EER Multiple Myeloma Panel by FISH|
- multiple myeloma
- Plasma cell neoplasms