Ordering Recommendation

May be useful for the evaluation of patients with progressive proximal muscle weakness and/or cutaneous manifestations suggestive of dermatomyositis and/or associated connective tissue disease.

Mnemonic
COMBI PAN
Methodology

Qualitative Immunoprecipitation/Semi-Quantitative Multiplex Bead Assay/Qualitative Immunoblot

Performed

Sun-Sat

Reported

7-18 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Serum Separator Tube (SST).

Specimen Preparation

Separate from cells ASAP or within 2 hours of collection. Transfer two 1 mL serum aliquots to ARUP Standard Transport Tubes. (Min: 0.5 mL/aliquot)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Hemolyzed, hyperlipemic, icteric, heat-treated or contaminated specimens.

Remarks
Stability

Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year

Reference Interval
Test Number
Components
Reference Interval
0099592 Jo-1 Antibody, IgG
29 AU/mL or less Negative
30-40 AU/mL Equivocal
41 AU/mL or greater Positive

  Mi-2 (nuclear helicase protein) Antibody Negative
  PL-7 (threonyl-tRNA synthetase) Antibody Negative
  PL-12 (alanyl-tRNA synthetase) Antibody Negative
  EJ (glycyl-tRNA synthetase) Antibody Negative
  SRP (Signal Recognition Particle) Ab Negative
  OJ (isoleucyl-tRNA synthetase) Antibody Negative
  P155/140 Antibody Negative
  SAE1 (SUMO activating enzyme) Ab Negative
  MDA5 (CADM-140) Ab Negative
  NXP2 (Nuclear matrix protein-2) Ab Negative
  TIF-1 gamma (155 kDa) Ab Negative

Interpretive Data

Refer to report.

Compliance Statement D: For laboratory tests using a manufactured RUO kit. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

Note

Antibodies:  PL-7, PL12, EJ, OJ, SRP, Jo-1, Mi-2, P155/140, SAE1, MDA5, NXP2, TIF1-gamma

Hotline History
N/A
CPT Codes

83516 x11; 86235

Components
Component Test Code* Component Chart Name LOINC
0099592 Jo-1 (Histidyl-tRNA Synthetase) Ab, IgG 33571-1
2010852 Mi-2 (nuclear helicase protein) Antibody 18485-3
2010853 PL-7 (threonyl-tRNA synthetase) Antibody 33772-5
2010854 PL-12 (alanyl-tRNA synthetase) Antibody 33771-7
2010855 P155/140 Antibody
2010856 EJ (glycyl-tRNA synthetase) Antibody 45149-2
2010859 SRP (Signal Recognition Particle) Ab 33921-8
2010860 OJ (isoleucyl-tRNA synthetase) Antibody 45152-6
2013997 Myositis Interpretive Information 48767-8
3001786 SAE1 (SUMO activating enzyme) Ab 82440-9
3001787 MDA5 (CADM-140) Ab 82424-3
3001788 NXP2 (Nuclear matrix protein-2) Ab 82426-8
3001789 TIF-1 gamma (155 kDa) Ab 82448-2
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
Dermatomyositis and Polymyositis Panel