Leukemia/Lymphoma Phenotyping Evaluation by Flow Cytometry

Flow cytometric immunophenotyping can aid in lineage assignment of acute leukemias and assist in diagnosis and classification of leukemias and lymphomas.

A panel of markers is performed on each case depending on the clinical history and specimen type. Without prior history or additional information, peripheral blood specimens typically undergo a preliminary analysis for B, T, NK, and myeloid/monocytic abnormalities, while bone marrows and lymph nodes undergo additional analysis for plasma cell abnormalities. Additional markers will be run as needed to further characterize abnormal populations.

Available Markers by population*:
T cell: CD1a, CD2, CD3, CD4, CD5, CD7, CD8, CD10, CD25, CD26, CD30, CD200, CD279 (PD-1), TCR gamma-delta, TRBC1, Cytoplasmic CD3
B cell: CD5, CD10, CD11c, CD19, CD20, CD22, CD23, CD25, CD38, CD103, CD123, CD200, surface Kappa, surface Lambda, cytoplasmic Kappa, cytoplasmic Lambda
Plasma cell: CD13, CD19, CD20, CD27, CD33, CD38, CD45, CD56, CD81, CD117, CD138, CD200, cytoplasmic Kappa, cytoplasmic Lambda
Myeloid: CD11b, CD13, CD14, CD15, CD16, CD33, CD34, CD36, CD38, CD41, CD42b, CD45, CD56, CD57, CD61, CD64, CD66b, CD71, CD117, CD123, HLA-DR, myeloperoxidase, glycophorin (CD235a), TdT
B lymphoblasts: CD10, CD19, CD20, CD22, CD24, CD38, CD45, CD58, CLRF2, cCD22, cCD79a
Mast Cell: CD2, CD25, CD30, CD117, CD123

*Not all markers will be reported in all cases. Requests for specific markers to be run must be listed on manual requisition or by footnote for electronic orders. We do not offer individual marker identification separately outside of the markers in this panel.

The report will include a pathologist interpretation and a marker interpretation range corresponding to CPT codes of 2-8 markers, 9-15markers, and 16+ markers interpreted. Charges apply per marker.