Time Sensitive
Ordering Recommendation

Primarily for evaluating lymphocyte function in patients with suspected cellular immune dysfunction, such as primary and secondary immunodeficiencies. Other uses include monitoring lymphocyte recovery and competence after hematopoietic stem cell transplantation and monitoring lymphocyte function during immunosuppressive therapy.


Cell Culture/Flow Cytometry


Thu, Fri


9-10 days

New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible.
Specimen Required
Patient Preparation

Collect control specimen from a healthy individual unrelated to patient.
Patient and control specimens must be collected ONLY on Wednesdays or Thursdays AND shipped directly to ARUP the same calendar day to meet the strict 48-hour stability requirement.


Green (sodium heparin) (patient) AND green (sodium heparin) (control) only on Wednesdays or Thursdays.

Specimen Preparation

Transport 10 mL whole blood (patient) AND 10 mL whole blood (control) in original collection tubes. (Min: 7 mL (patient) AND 7 mL (control)). Infant Minimum: 3 mL (patient AND 7 mL (control).
Do not refrigerate or freeze. LIVE LYMPHOCYTES REQUIRED.

Storage/Transport Temperature

Must be collected and shipped directly to ARUP the same calendar day.

Unacceptable Conditions

Refrigerated or frozen specimens.


Do not collect or ship on, or the day before, holidays.


Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable

Reference Interval
CD45 Pos Cells 39.7% or greater 15.5% or greater 10.0% or greater
CD3 Pos Cells 42.5% or greater 17.4% or greater 8.0% or greater
CD19 Pos Cells 51.9% or greater N/A 6.3% or greater

Interpretive Data

This test measures lymphocyte proliferation in response to stimulation with nonspecific mitogens phytohemagglutinin (PHA), concanavalin A (Con A), and pokeweed mitogen (PWM), determined by flow cytometry. Proliferating cells are detected by fluorescent labeling. Results are reported as percent proliferating cells of total specific cell populations.

Compliance Statement B: For laboratory developed tests not using a RUO kit, and for FDA approved, cleared or 510(k) exempt assays with alterations. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

Hotline History
CPT Codes

86353 x3

Component Test Code* Component Chart Name LOINC
3001322 CD45+ Cell Proliferation by PHA 69038-8
3001323 CD3+ Cell Proliferation by PHA 69013-1
3001324 CD19+ Cell Proliferation by PHA
3001325 CD45+ Cell Proliferation by Con A
3001326 CD3+ Cell Proliferation by Con A
3001327 CD45+ Cell Proliferation by PWM 69019-8
3001328 CD3+ Cell Proliferation by PWM 69020-6
3001329 CD19+ Cell Proliferation by PWM 69037-0
3001336 Lymphocyte Proliferation-Mitogen, Interp 53810-8
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Lymphocyte Proliferation, Mitogen Induced, by Flow Cytometry (48-Hr Critical Room Temp)