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Time Sensitive
Ordering Recommendation

Primarily used for research and to support attempts to understand the pathogenesis of immune, infectious, allergic, or inflammatory disorders.

Mnemonic
LMP
Methodology

Cell Culture

Performed

Tue-Fri

Reported

9-10 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation

Collect control specimen from a healthy individual unrelated to patient.

Collect

Green (sodium heparin) (patient) AND green (sodium heparin) (control). Also acceptable: Yellow (ACD solution A) (patient) AND yellow (ACD solution A) (control). Patient and control specimens must be collected within 48 hours of test performance.

Specimen Preparation

Transport 10 mL whole blood (patient) AND 10 mL whole blood (control) in original collection tubes. (Min: 7 mL (patient) AND 7 mL (control)) Do not refrigerate or freeze. LIVE LYMPHOCYTES REQUIRED.
Infant Minimum: 3 mL (patient) AND 7 mL (control).

Storage/Transport Temperature

CRITICAL ROOM TEMPERATURE.

Unacceptable Conditions

Refrigerated or frozen specimens. Specimens in transport longer than 48 hours.

Remarks
Stability

Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
New York State Clients: Ambient: 24 hours; Refrigerated: Unacceptable; Frozen: Unacceptable

Reference Interval

By report

Interpretive Data

Lymphocyte proliferation in response to the nonspecific mitogens phytohemagglutinin (PHA), concanavalin A (Con A), and pokeweed are determined by 3H-thymidine incorporation.

Results are reported as counts per minute (CPM) mitogen stimulated versus a control culture and a stimulation index (SI) which represents the ratio of CPM of the stimulated lymphocytes to the mean CPM of the unstimulated control. 

Compliance Category

Laboratory Developed Test (LDT)

Note

Interpretation comparing the patient results to the client normal control and the laboratory control will be provided by the medical director.

Hotline History
N/A
CPT Codes

86353 x3

Components
Component Test Code* Component Chart Name LOINC
0096044 Lymphocyte Mitogen Proliferation 58722-0
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Mitogen Proliferation, Lymphocytes
Lymphocyte Mitogen Proliferation