Ordering Recommendation
Primarily used for research and to support attempts to understand the pathogenesis of immune, infectious, allergic, or inflammatory disorders.
Mnemonic
Methodology
Cell Culture
Performed
Tue-Fri
Reported
9-10 days
New York DOH Approval Status
Specimen Required
Collect control specimen from a healthy individual unrelated to patient.
Green (sodium heparin) (patient) AND green (sodium heparin) (control). Also acceptable: Yellow (ACD solution A) (patient) AND yellow (ACD solution A) (control). Patient and control specimens must be collected within 48 hours of test performance.
Transport 10 mL whole blood (patient) AND 10 mL whole blood (control) in original collection tubes. (Min: 7 mL (patient) AND 7 mL (control)) Do not refrigerate or freeze. LIVE LYMPHOCYTES REQUIRED.
Infant Minimum: 3 mL (patient) AND 7 mL (control).
CRITICAL ROOM TEMPERATURE.
Refrigerated or frozen specimens. Specimens in transport longer than 48 hours.
Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
New York State Clients: Ambient: 24 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
Reference Interval
By report
Interpretive Data
Lymphocyte proliferation in response to the nonspecific mitogens phytohemagglutinin (PHA), concanavalin A (Con A), and pokeweed are determined by 3H-thymidine incorporation.
Results are reported as counts per minute (CPM) mitogen stimulated versus a control culture and a stimulation index (SI) which represents the ratio of CPM of the stimulated lymphocytes to the mean CPM of the unstimulated control.
This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.
Laboratory Developed Test (LDT)
Note
Interpretation comparing the patient results to the client normal control and the laboratory control will be provided by the medical director.
Hotline History
CPT Codes
86353 x3
Components
Component Test Code* | Component Chart Name | LOINC |
---|---|---|
0096044 | Lymphocyte Mitogen Proliferation | 53810-8 |
Aliases
- Mitogen Proliferation, Lymphocytes