Lymphocyte Proliferation, Antigen Induced, by Flow Cytometry (24-Hr Critical Room Temp)

3001320
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Time Sensitive

Ordering Recommendation

Primarily for evaluating recall antigen responses in patients with suspected cellular immune dysfunction, such as primary and secondary immunodeficiencies. Other uses include monitoring lymphocyte recovery and competence after hematopoietic stem cell transplantation and monitoring lymphocyte function during immunosuppressive therapy. Do not order for patients younger than 3 months unless clinical history of candidiasis is present.

Mnemonic

LPA FLOW

Methodology

Cell Culture/Flow Cytometry

Performed

Thu, Fri

Reported

9-10 days

New York DOH Approval Status

Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible.

Specimen Required

Patient Preparation

Collect control specimen from a healthy individual unrelated to patient.
Patient and control specimens must be collected ONLY on Wednesdays or Thursdays AND shipped directly to ARUP the same calendar day to meet the strict 24-hour stability requirement.

Collect

Green (sodium heparin) (patient) AND green (sodium heparin) (control) only on Wednesdays or Thursdays.

Specimen Preparation

Transport 10 mL whole blood (patient) AND 10 mL whole blood (control) in original collection tubes. (Min: 7 mL (patient) AND 7 mL (control)). Infant Minimum: 3 mL (patient) AND 7 mL (control).
Do not refrigerate or freeze. LIVE LYMPHOCYTES REQUIRED.

Storage/Transport Temperature

CRITICAL ROOM TEMPERATURE.
Must be collected and shipped directly to ARUP the same calendar day.

Unacceptable Conditions

Refrigerated or frozen specimens.

Remarks

Do not collect or ship on, or the day before, holidays.

Stability

Ambient: 24 hours; Refrigerated: Unacceptable; Frozen: Unacceptable

Reference Interval

Tetanus Candida
CD45 Pos Cells 2.9% or greater 9.6% or greater
CD3 Pos Cells 3.0% or greater 2.8% or greater

Interpretive Data

This test measures T lymphocyte proliferation in response to stimulation with recall antigens tetanus toxoid and Candida, determined by flow cytometry. Proliferating cells are detected by fluorescent labeling. Results are reported as percent proliferating cells of total specific cell populations.

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Laboratory Developed Test (LDT)

Note

Hotline History

N/A

CPT Codes

86353 x2

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Components

Component Test Code* Component Chart Name LOINC
3001330 CD45+ Cell Proliferation by Tetanus 69016-4
3001331 CD3+ Cell Proliferation by Tetanus 69029-7
3001332 CD45+ Cell Proliferation by Candida 69014-9
3001333 CD3+ Cell Proliferation by Candida 69015-6
3001335 Lymphocyte Proliferation-Antigen, Interp 53810-8
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

Lymphocyte Proliferation, Antigen Induced, by Flow Cytometry (24-Hr Critical Room Temp)