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Lymphocyte Antigen Proliferation
Ordering Recommendation
Do not order for patients younger than 3 months unless clinical history of candidiasis is present. For patients who are lymphopenic, collection of 10 mL of whole blood, rather than the minimum required volumes, is recommended to increase the likelihood that a sufficient number of lymphocytes can be isolated for testing.
New York DOH Approval Status
Specimen Required
Collect control specimen from a healthy individual unrelated to patient at approximately the same time as and under similar conditions to the patient.
Green (sodium heparin) (patient) AND green (sodium heparin) (control). Also acceptable: Yellow (ACD solution A) (patient) AND yellow (ACD solution A) (control). Patient and control specimens must be collected within 48 hours of test performance.
Transport 10 mL whole blood (patient) AND 10 mL whole blood (control) in original collection tubes. (Min: 7 mL (patient) AND 7 mL (control)). Do not refrigerate or freeze. LIVE LYMPHOCYTES REQUIRED.
Infant Minimum: 3 mL (patient) AND 7 mL (control).
For patients who are lymphopenic, collection of 10 mL of whole blood, rather than the minimum required volumes, is recommended to increase the likelihood that a sufficient number of lymphocytes can be isolated for testing.
CRITICAL ROOM TEMPERATURE.
Yellow (ACD solution B). Refrigerated or frozen specimens. Specimens in transport longer than 48 hours.
Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
New York State Clients: Ambient: 24 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
Methodology
Cell Culture
Performed
Sun, Tue, Wed, Thu, Fri, Sat
Reported
9-10 days
Reference Interval
By report
Interpretive Data
Candida and tetanus antigens are tested independently in lymphocyte culture. Lymphocyte proliferation in response to these antigens is determined by 3H -thymidine incorporation.
Results are reported as the counts per minute (CPM) antigen stimulated versus control cultures and a stimulation index (SI) which represents the ratio of mean CPM of the stimulated lymphocytes to the mean CPM of the unstimulated control.
Laboratory Developed Test (LDT)
Note
Interpretation comparing the patient results to the client normal control and the laboratory control will be provided by the medical director.
Hotline History
CPT Codes
86353 x2
Components
| Component Test Code* | Component Chart Name | LOINC |
|---|---|---|
| 0096055 | Lymphocyte Ag Proliferation |
Aliases
- Antigen Proliferation, Lymphocytes
