Time Sensitive

Ordering Recommendation

Do not order for patients younger than 3 months unless clinical history of candidiasis is present. For patients who are lymphopenic, collection of 10 mL of whole blood, rather than the minimum required volumes, is recommended to increase the likelihood that a sufficient number of lymphocytes can be isolated for testing.


Cell Culture


Sun, Tue, Wed, Thu, Fri, Sat


9-10 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation

Collect control specimen from a healthy individual unrelated to patient at approximately the same time as and under similar conditions to the patient.


Green (sodium heparin) (patient) AND green (sodium heparin) (control). Also acceptable: Yellow (ACD Solution A) (patient) AND yellow (ACD Solution A) (control). Patient and control specimens must be collected within 48 hours of test performance.

Specimen Preparation

Transport 10 mL whole blood (patient) AND 10 mL whole blood (control) in original collection tubes. (Min: 7 mL (patient) AND 7 mL (control)) Do not refrigerate or freeze. LIVE LYMPHOCYTES REQUIRED.
Infant Minimum: 3 mL (patient) AND 7 mL (control).

Storage/Transport Temperature


Unacceptable Conditions

Yellow (ACD Solution B).  Refrigerated or frozen specimens. Specimens in transport longer than 48 hours.


Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
New York State Clients: Ambient: 24 hours; Refrigerated: Unacceptable; Frozen: Unacceptable

Reference Interval

By report

Interpretive Data

Candida and tetanus antigens are tested independently in lymphocyte culture. Lymphocyte proliferation in response to these antigens is determined by 3H-thymidine incorporation.

Results are reported as the counts per minute (CPM) antigen stimulated versus control cultures and a stimulation index (SI) which represents the ratio of mean CPM of the stimulated lymphocytes to the mean CPM of the unstimulated control.

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Laboratory Developed Test (LDT)


Interpretation comparing the patient results to the client normal control and the laboratory control will be provided by the medical director.

Hotline History


CPT Codes

86353 x2


Component Test Code* Component Chart Name LOINC
0096055 Lymphocyte Ag Proliferation
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • Antigen Proliferation, Lymphocytes
Lymphocyte Antigen Proliferation