Icon

Time Sensitive
Ordering Recommendation

Do not order for patients younger than 3 months unless clinical history of candidiasis is present.

Mnemonic
LAPP
Methodology

Cell Culture

Performed

Tue-Fri

Reported

9-10 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation

Collect control specimen from a healthy individual unrelated to patient at approximately the same time as and under similar conditions to the patient.

Collect

Green (sodium heparin) (patient) AND green (sodium heparin) (control). Also acceptable: Yellow (ACD Solution A) (patient) AND yellow (ACD Solution A) (control). Patient and control specimens must be collected within 48 hours of test performance.

Specimen Preparation

Transport 10 mL whole blood (patient) AND 10 mL whole blood (control) in original collection tubes. (Min: 7 mL (patient) AND 7 mL (control)) Do not refrigerate or freeze. LIVE LYMPHOCYTES REQUIRED.
Infant Minimum: 3 mL (patient) AND 7 mL (control).

Storage/Transport Temperature

CRITICAL ROOM TEMPERATURE.

Unacceptable Conditions

Yellow (ACD Solution B).  Refrigerated or frozen specimens. Specimens in transport longer than 48 hours.

Remarks
Stability

Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
New York State Clients: Ambient: 24 hours; Refrigerated: Unacceptable; Frozen: Unacceptable

Reference Interval

By report

Interpretive Data

Candida and tetanus antigens are tested independently in lymphocyte culture. Lymphocyte proliferation in response to these antigens is determined by 3H-thymidine incorporation.

Results are reported as the counts per minute (CPM) antigen stimulated versus control cultures and a stimulation index (SI) which represents the ratio of mean CPM of the stimulated lymphocytes to the mean CPM of the unstimulated control.

Compliance Category

Laboratory Developed Test (LDT)

Note

Interpretation comparing the patient results to the client normal control and the laboratory control will be provided by the medical director.

Hotline History
N/A
CPT Codes

86353 x2

Components
Component Test Code* Component Chart Name LOINC
0096055 Lymphocyte Ag Proliferation
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Antigen Proliferation, Lymphocytes
Lymphocyte Antigen Proliferation