Time Sensitive

Ordering Recommendation

Primarily for evaluating lymphocyte function in patients with suspected cellular immune dysfunction, such as primary and secondary immunodeficiencies. Other uses include monitoring lymphocyte recovery and competence after hematopoietic stem cell transplantation and monitoring lymphocyte function during immunosuppressive therapy. Do not order for patients younger than 3 months unless clinical history of candidiasis is present.




Cell Culture/Flow Cytometry


Thu, Fri


9-10 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation

Collect control specimen from a healthy individual unrelated to patient.
Patient and control specimens must be collected ONLY on Wednesdays or Thursdays AND shipped directly to ARUP the same calendar day to meet the strict 24-hour stability requirement.


Green (sodium heparin) (patient) AND green (sodium heparin) (control) only on Wednesdays or Thursdays.

Specimen Preparation

Transport 10 mL whole blood (patient) AND 10 mL whole blood (control) in original collection tubes. (Min: 7 mL (patient) AND 7 mL (control)). Infant Minimum: 3 mL (patient) AND 7 mL (control).
Do not refrigerate or freeze. LIVE LYMPHOCYTES REQUIRED.

Storage/Transport Temperature

Must be collected and shipped directly to ARUP the same calendar day.

Unacceptable Conditions

Refrigerated or frozen specimens.


Do not collect or ship on, or the day before, holidays.


Ambient: 24 hours; Refrigerated: Unacceptable; Frozen: Unacceptable

Reference Interval

CD45 Pos Cells 39.7% or greater 15.5% or greater 10.0% or greater
CD3 Pos Cells 42.5% or greater 17.4% or greater 8.0% or greater
CD19 Pos Cells 51.9% or greater N/A 6.3% or greater
Tetanus Candida
CD45 Pos Cells 2.9% or greater 9.6% or greater
CD3 Pos Cells 3.0% or greater 2.8% or greater

Interpretive Data

This test measures lymphocyte proliferation in response to stimulation with nonspecific mitogens phytohemagglutinin (PHA), concanavalin A (Con A), and pokeweed mitogen (PWM); and T lymphocyte proliferation in response to stimulation with recall antigens tetanus toxoid and Candida, determined by flow cytometry. Proliferating cells are detected by fluorescent labeling. Results are reported as percent proliferating cells of total specific cell populations.

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Laboratory Developed Test (LDT)


Hotline History


CPT Codes

86353 x5


Component Test Code* Component Chart Name LOINC
3001322 CD45+ Cell Proliferation by PHA 69038-8
3001323 CD3+ Cell Proliferation by PHA 69013-1
3001324 CD19+ Cell Proliferation by PHA
3001325 CD45+ Cell Proliferation by Con A
3001326 CD3+ Cell Proliferation by Con A
3001327 CD45+ Cell Proliferation by PWM 69019-8
3001328 CD3+ Cell Proliferation by PWM 69020-6
3001329 CD19+ Cell Proliferation by PWM 69037-0
3001330 CD45+ Cell Proliferation by Tetanus 69016-4
3001331 CD3+ Cell Proliferation by Tetanus 69029-7
3001332 CD45+ Cell Proliferation by Candida 69014-9
3001333 CD3+ Cell Proliferation by Candida 69015-6
3001334 Lymphocyte Antigen Mitogen Panel Interp 53810-8
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


Lymphocyte Proliferation, Antigen-Mitogen Panel by Flow Cytometry (24-Hr Critical Room Temp)