Evaluate patients with suspected immunodeficiency diseases, including severe combined immunodeficiency. Do not order for patients younger than 3 months unless clinical history of candidiasis is present.
Collect control specimen from a healthy individual unrelated to patient at approximately the same time as and under similar conditions to the patient.
Green (sodium heparin) (patient) AND green (sodium heparin) (control). Also acceptable: Yellow (ACD Solution A) (patient) AND yellow (ACD Solution A) (control). Patient and control specimens must be collected within 48 hours of test performance.
Transport 10 mL whole blood (patient) AND 10 mL whole blood (control) in original collection tubes. (Min: 7 mL (patient) AND 7 mL (control)) Do not refrigerate or freeze. LIVE LYMPHOCYTES REQUIRED.
Infant Minimum: 3 mL (patient) AND 7 mL (control).
CRITICAL ROOM TEMPERATURE.
Yellow (ACD Solution B). Refrigerated or frozen specimens. Specimens in transport longer than 48 hours.
Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
New York State Clients: Ambient 24 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
Phytohemagglutinin, concanavalin A, pokeweed mitogen, Candida antigen, and tetanus antigen are tested independently in lymphocyte culture. Lymphocyte proliferation in response to these mitogens and antigens is determined by 3H-thymidine incorporation.
Results are reported as the counts per minute (CPM) mitogen or antigen stimulated versus a control culture and a stimulation index (SI) which represents the ratio of CPM of the stimulated lymphocytes to the mean CPM of the unstimulated control.
Laboratory Developed Test (LDT)
Interpretation comparing the patient results to the client normal control and the laboratory control will be provided by the medical director.
|Component Test Code*||Component Chart Name||LOINC|
|0096056||Lymphocyte Ag and Mitogen Panel||59063-8|
- Antigen & Mitogen Proliferation Panel
- Blastogenesis Antigens
- Blastogenesis Mitogens
- Lymphocyte Blastogenesis
- Lymphocyte Blastogenesis Antigens
- Mitogen Studies